Williams P H, Rabsch W, Methner U, Voigt W, Tschäpe H, Reissbrodt R
Department of Genetics, University of Leicester, University Road, Leicester LE1 7RH, UK.
Vaccine. 2006 May 1;24(18):3840-4. doi: 10.1016/j.vaccine.2005.07.020. Epub 2005 Jul 25.
Three outer membrane proteins of Salmonella enterica serovar Typhimurium function as catecholate siderophore receptors. IroN promotes uptake of enterobactin, salmochelins and 2,3-dihydroxybenzoylserine, FepA transports enterobactin and 2,3-dihydroxybenzoylserine, and Cir is a receptor for 2,3-dihydroxybenzoylserine. In addition, all three proteins are required for l-norepinephrine-facilitated iron uptake from transferrin as judged by failure of a fepA iroN cir triple mutant to grow in serum-containing medium in the presence of l-norepinephrine. Moreover, pre-treatment of mice with l-norepinephrine resulted in enhanced systemic spread of the parental strain, but had no effect on the fepA iroN cir mutant. Inoculation of mice with the triple mutant, which is significantly attenuated, elicited a significant protective effect against subsequent challenge with the parental strain.
鼠伤寒沙门氏菌的三种外膜蛋白作为儿茶酚盐铁载体受体发挥作用。IroN促进肠杆菌素、沙门菌素和2,3-二羟基苯甲酰丝氨酸的摄取，FepA转运肠杆菌素和2,3-二羟基苯甲酰丝氨酸，而Cir是2,3-二羟基苯甲酰丝氨酸的受体。此外，根据fepA iroN cir三重突变体在含血清培养基中添加l-去甲肾上腺素时无法生长判断，这三种蛋白都是l-去甲肾上腺素促进从转铁蛋白摄取铁所必需的。此外，用l-去甲肾上腺素预处理小鼠导致亲本菌株的全身扩散增强，但对fepA iroN cir突变体没有影响。接种明显减毒的三重突变体小鼠对随后亲本菌株的攻击产生了显著的保护作用。
