Pasquali Paolo, Ammendola Serena, Pistoia Claudia, Petrucci Paola, Tarantino Michela, Valente Carlo, Marenzoni Maria Luisa, Rotilio Giuseppe, Battistoni Andrea
Department of Food Safety and Veterinary Public Health, Istituto Superiore di Sanità, 00161 Rome, Italy.
Vaccine. 2008 Jun 25;26(27-28):3421-6. doi: 10.1016/j.vaccine.2008.04.036. Epub 2008 May 5.
Salmonella enterica has long been recognised as an important zoonotic pathogen of economic significance, both in animals and humans. We have recently shown that inactivation of the ZnuABC high affinity zinc transporter significantly affects the pathogenicity of S. enterica, likely due to zinc shortage in the eukaryotic tissues. Here, we demonstrate that a S. enterica serovar Typhimurium znuABC deleted strain is able to induce a short lasting infection in mice. On the same time, it primes a cell-mediated immune response, which confers a solid and durable immune-based protection against challenge infections with virulent strains of S. Typhimurium. These findings suggest the possibility to explore the use of S. enterica ZnuABC deleted mutants for the production on novel vaccines.
肠炎沙门氏菌长期以来一直被认为是一种对动物和人类具有重要经济意义的重要人畜共患病原体。我们最近发现，ZnuABC高亲和力锌转运蛋白的失活会显著影响肠炎沙门氏菌的致病性，这可能是由于真核组织中锌缺乏所致。在此，我们证明肠炎沙门氏菌鼠伤寒血清型的znuABC缺失菌株能够在小鼠中引发短暂的感染。同时，它能引发细胞介导的免疫反应，这种反应能为抵抗鼠伤寒沙门氏菌强毒株的攻击感染提供坚实而持久的基于免疫的保护。这些发现提示了探索使用肠炎沙门氏菌ZnuABC缺失突变体来生产新型疫苗的可能性。
