Mizukoshi Genki, Katsura Ken-Ichiro, Katayama Yasuo
The Second Department of Internal Medicine, Nippon Medical School, Tokyo, Japan.
Neurol Res. 2005 Sep;27(6):644-6. doi: 10.1179/016164105X25153.
Our aim was to explore whether free radical damage against DNA exists or not, in human stroke patients.
Urinary 8-hyroxy-2'-deoxyguanosine (8-OHdG) and serum S100beta were measured in acute cardioembolic stroke patients with relatively severe symptoms. During days 3--5 after the onset of stroke, urine was collected for 24 hours for 8-OHdG measurement and serum was sampled for S100beta analysis every day.
The total urinary 8-OHdG content was significantly higher in stroke patients than in non-stroke inpatients (p<0.01). The average value was more than twice the value of that of non-stroke inpatients. The total urinary 8-OHdG contents showed significant correlation (r=0.87, p<0.01) with serum S100beta values, which are reportedly related to brain damage.
These findings suggest that urinary 8-OHdG in acute stroke patients may originate from brain tissue, and DNA damage also exists in the brain of stroke patients.
我们的目的是探究在人类中风患者中是否存在针对DNA的自由基损伤。
对症状相对严重的急性心源性栓塞性中风患者测定尿8-羟基-2'-脱氧鸟苷（8-OHdG）和血清S100β。在中风发作后的第3至5天，收集24小时尿液用于8-OHdG测量，每天采集血清用于S100β分析。
中风患者尿中8-OHdG的总含量显著高于非中风住院患者（p<0.01）。平均值是非中风住院患者的两倍多。尿中8-OHdG的总含量与血清S100β值呈显著相关性（r = 0.87，p<0.01），据报道血清S100β值与脑损伤有关。
这些发现表明急性中风患者尿中的8-OHdG可能源自脑组织，中风患者的大脑中也存在DNA损伤。
