Chang Chen-Kai, Chang Ku-Shang, Lin Yi-Chen, Liu Sin-Yie, Chen Chien-Yuan
Institute of Microbiology and Biochemistry, National Taiwan University, Taipei, Taiwan.
Biotechnol Lett. 2005 Aug;27(16):1165-9. doi: 10.1007/s10529-005-8653-7.
Hairy root cultures of Gynostemma pentaphyllum were established by infecting leaf discs with Agrobacterium rhizogenes. The dry biomass of hairy roots grown in MS medium for 49 days was 7.3 g l(-1) with a gypenoside content of 38 mg g(-1) dry wt.
通过用发根农杆菌感染绞股蓝叶片圆盘建立了绞股蓝毛状根培养物。在MS培养基中生长49天的毛状根干生物量为7.3 g l(-1)，绞股蓝皂苷含量为38 mg g(-1)干重。
