Ho Yi-Ping, Kung Matthew C, Yang Samuel, Wang Tza-Huei
Department of Mechanical Engineering, The Johns Hopkins University and School of Medicine, Baltimore, Maryland 21218, USA.
Nano Lett. 2005 Sep;5(9):1693-7. doi: 10.1021/nl050888v.
We demonstrate a hybridization detection method using multicolor oligonucleotide-functionalized quantum dots as nanoprobes. In the presence of various target sequences, combinatorial self-assembly of the nanoprobes via independent hybridization reactions leads to the generation of discernible sequence-specific spectral codings. Detection of single-molecule hybridization is achieved by measuring colocalization of individual nanoprobes. Genetic analysis for anthrax pathogenicity through simultaneous detection of multiple relevant sequences is demonstrated using this novel biosensing method as proof-of-concept.
我们展示了一种使用多色寡核苷酸功能化量子点作为纳米探针的杂交检测方法。在存在各种靶序列的情况下，纳米探针通过独立的杂交反应进行组合自组装，从而产生可分辨的序列特异性光谱编码。通过测量单个纳米探针的共定位来实现单分子杂交的检测。作为概念验证，使用这种新型生物传感方法展示了通过同时检测多个相关序列对炭疽致病性进行基因分析。
