Abdurakhmonov I Y, Abdullaev A A, Saha S, Buriev Z T, Arslanov D, Kuryazov Z, Mavlonov G T, Rizaeva S M, Reddy U K, Jenkins J N, Abdullaev A, Abdukarimov A
Laboratory of Genetic Engineering and Biotechnology, Institute of Genetics and Plant Experimental Biology, Academy of Sciences of Uzbekistan, Yuqori Yuz, Qibray Region, Tashkent District, 702151 Uzbekistan.
J Hered. 2005 Nov-Dec;96(6):644-53. doi: 10.1093/jhered/esi097. Epub 2005 Sep 13.
Cotton (Gossypium hirsutum L.) leaf defoliation has a significant ecological and economical impact on cotton production. Thus the utilization of a natural leaf defoliation trait, which exists in wild diploid cotton species, in the development of tetraploid cultivated cotton will not only be cost effective, but will also facilitate production of very high-grade fiber. The primary goal of our research was to tag loci associated with natural leaf defoliation using microsatellite markers in Upland cotton. The F2 populations developed from reciprocal crosses between the two parental cotton lines--AN-Boyovut-2 (2n = 52), a late leaf defoliating type, and Listopad Beliy (2n = 52), a naturally early leaf defoliating type--demonstrated that the naturally early leaf defoliation trait has heritability values of 0.74 and 0.84 in the reciprocal F2 population. The observed phenotypic segregation difference in reciprocal crosses suggested a minor cytoplasmic effect in the phenotypic expression of the naturally early leaf defoliation trait. Results from the Kruskal-Wallis (KW) nonparametric test revealed that JESPR-13 (KW = 6.17), JESPR-153 (KW = 9.97), and JESPR-178 (KW = 13.45) Simple sequence repeat (SSR) markers are significantly associated with natural leaf defoliation in the mapping population having stable estimates at empirically obtained critical thresholds (P < .05-.0001). JESPR-178 revealed the highest estimates (P < .0001) for association with the natural leaf defoliation trait, exceeding maximum empirical threshold values. JESPR-178 was assigned to the short arm of chromosome 18, suggesting indirectly that genes associated with natural leaf defoliation might be located on this chromosome. This microsatellite marker may have the potential for use to introgress the naturally early leaf defoliation quantitative trait loci (QTL) from the donor line Listopad Beliy to commercial varieties of cotton through marker-assisted selection programs.
棉花（陆地棉）叶片脱落对棉花生产具有重大的生态和经济影响。因此，在四倍体栽培棉花的培育过程中利用野生二倍体棉花物种中存在的自然叶片脱落特性，不仅具有成本效益，还将有助于生产出高品质的纤维。我们研究的主要目标是利用微卫星标记在陆地棉中标记与自然叶片脱落相关的基因座。由两个亲本棉花品系——AN - Boyovut - 2（2n = 52），一种晚落叶类型，和Listopad Beliy（2n = 52），一种自然早落叶类型——之间的正反交产生的F2群体表明，自然早落叶性状在正反交F2群体中的遗传力值分别为0.74和0.84。正反交中观察到的表型分离差异表明，自然早落叶性状的表型表达存在较小的细胞质效应。Kruskal - Wallis（KW）非参数检验结果显示，JESPR - 13（KW = 6.17）、JESPR - 153（KW = 9.97）和JESPR - 178（KW = 13.45）简单序列重复（SSR）标记与定位群体中的自然叶片脱落显著相关，在经验获得的临界阈值（P <.05 -.0001）下具有稳定的估计值。JESPR - 178与自然叶片脱落性状关联的估计值最高（P <.0001），超过了最大经验阈值。JESPR - 178被定位到第18号染色体的短臂上，这间接表明与自然叶片脱落相关的基因可能位于该染色体上。这种微卫星标记可能有潜力通过标记辅助选择程序，将供体品系Listopad Beliy中的自然早落叶数量性状基因座（QTL）导入棉花商业品种中。
