Nishikawa Tetsunari, Masuno Kazuya, Tominaga Kazuya, Koyama Yoshihisa, Yamada Takeki, Takakuda Kazuo, Kikuchi Masanori, Tanaka Junzo, Tanaka Akio
Oral Pathology, Osaka Dental University, 8-1 Kuzuha hanazono-cyo, Hirakata-shi, Osaka 573-1121, Japan.
Implant Dent. 2005 Sep;14(3):252-60. doi: 10.1097/01.id.0000173628.00705.d0.
The purpose of this study was to evaluate a biodegradable hydroxyapatite/collagen composite and to examine the use of the calcium ion contained for bone formation and growth. Surgical holes were prepared in the femora and tibiae of beagle dogs, and were filled with the hydroxyapatite/collagen composite labeled with alizarin red. After 4 weeks, calcein was administered to the experimental dogs. After 1 additional week, the femora and tibiae were removed surgically and fixed in formalin. Light microscopy and confocal laser scanning microscopy were used to examine the surgical holes with their implanted materials and the surrounding bone. There were only a few inflammatory cells adjacent to the hydroxyapatite/collagen composite. The newly formed bone in the cortical bone was stained with calcein, which binds to serum calcium, and new bone near the hydroxyapatite/collagen composite in the holes was stained positive for alizarin red, which binds to the calcium in the hydroxyapatite/collagen composite. In addition, osteoblasts near the hydroxyapatite/collagen composite as well as newly formed bone adjacent to the osteoblasts showed alizarin red staining, but the new bone at a distance from the hydroxyapatite/collagen implant reacted only to calcein staining. These results, using the tissue labeling method with calcein and alizarin red, suggested that the calcium bound to the alizarin red released from the hydroxyapatite/collagen composite materials might have been translocated to sites of new bone formation. The present experiment showed that the novel hydroxyapatite/collagen composite is a useful implant material for bone augmentation and that the calcium in the newly formed bone might have been released from the implant.
本研究的目的是评估一种可生物降解的羟基磷灰石/胶原蛋白复合材料，并研究其中所含钙离子在骨形成和生长中的作用。在比格犬的股骨和胫骨上制备手术孔，并用茜素红标记的羟基磷灰石/胶原蛋白复合材料填充。4周后，给实验犬注射钙黄绿素。再过1周后，手术切除股骨和胫骨并固定于福尔马林中。使用光学显微镜和共聚焦激光扫描显微镜检查带有植入材料的手术孔及其周围的骨组织。羟基磷灰石/胶原蛋白复合材料附近仅有少量炎性细胞。皮质骨中新形成的骨被与血清钙结合的钙黄绿素染色，而孔中羟基磷灰石/胶原蛋白复合材料附近的新骨被与羟基磷灰石/胶原蛋白复合材料中的钙结合的茜素红染成阳性。此外，羟基磷灰石/胶原蛋白复合材料附近的成骨细胞以及与成骨细胞相邻的新形成的骨均显示茜素红染色，但远离羟基磷灰石/胶原蛋白植入物的新骨仅对钙黄绿素染色有反应。这些使用钙黄绿素和茜素红组织标记方法的结果表明，从羟基磷灰石/胶原蛋白复合材料中释放的与茜素红结合的钙可能已转移至新骨形成部位。本实验表明，新型羟基磷灰石/胶原蛋白复合材料是一种用于骨增量的有用植入材料，并且新形成骨中的钙可能已从植入物中释放。
