Njemini Rose, Lambert Margareta, Demanet Christian, Mets Tony
Geriatric Unit, Academic Hospital, Vrije Universiteit Brussel, Belgium.
J Clin Immunol. 2005 Sep;25(5):405-17. doi: 10.1007/s10875-005-5361-y.
The purpose of the present study was to assess the influence of age and acute infection on the production of Hsp32 in human peripheral blood cells, using flow cytometry. Thirty-five controls and 31 patients with acute infection participated. We found that the age and inflammatory status correlated positively with Hsp32 levels in both heat shocked (HS) and non-HS monocytes and lymphocytes. In addition, the HS response of Hsp32 was different in these peripheral blood cells; whereas HS exerted an up-regulation in the levels of Hsp32 in monocytes, a significant decrease in Hsp32 levels was noticed for lymphocytes. We found significant relationships between circulating C-reactive protein as well as interleukin-6 and the levels of Hsp32 in cells. We conclude that Hsp32 is up-regulated in the elderly as well as in individuals with inflammation, and that the HS response of Hsp32 is different in monocytes as compared to lymphocytes.
本研究的目的是使用流式细胞术评估年龄和急性感染对人外周血细胞中Hsp32产生的影响。35名对照者和31名急性感染患者参与了研究。我们发现，在热休克（HS）和非HS单核细胞及淋巴细胞中，年龄和炎症状态均与Hsp32水平呈正相关。此外，这些外周血细胞中Hsp32的HS反应有所不同；HS使单核细胞中Hsp32水平上调，而淋巴细胞中Hsp32水平则显著下降。我们发现循环C反应蛋白以及白细胞介素-6与细胞中Hsp32水平之间存在显著关联。我们得出结论，Hsp32在老年人以及有炎症的个体中上调，并且与淋巴细胞相比，单核细胞中Hsp32的HS反应有所不同。
