Njemini R, Lambert M, Demanet C, Mets T
Geriatric Unit, Academic Hospital, Vrije Universiteit Brussel (VUB), Laarbeeklaan 101, B-1090 Brussels, Belgium.
Exp Gerontol. 2006 Mar;41(3):312-9. doi: 10.1016/j.exger.2006.01.006. Epub 2006 Feb 28.
Heat shock proteins (Hsp) are highly conserved proteins and their synthesis is ubiquitous in virtually every species in which they have been sought. In the present study we have investigated the effect of age and inflammation on the induction of Hsp27 in human peripheral blood mononuclear cells, using flow cytometry. Sixty-six healthy control subjects or patients suffering from inflammation participated in the study. In both heat shocked (HS) and non-HS conditions, the percentage of Hsp27 producing lymphocytes as well as the intensity of Hsp27 in lymphocytes and monocytes were negatively influenced by age. The basal levels and also the levels of Hsp27 production after HS were higher for monocytes compared to lymphocytes. In addition, we found that HS resulted in a small but significant increase in the levels of Hsp27 in lymphocytes whereas a significant decrease in Hsp27 was noticed for monocytes. In conclusion, results presented herein provide evidence in support of an age-related decrease in the level of Hsp27, which disappeared in the presence of inflammation. Several relationships between the circulating levels of CRP, IL-6 and TNF-alpha with the various Hsp27 determinations were observed, indicating that cytokines are able to influence the production of Hsp27.
热休克蛋白（Hsp）是高度保守的蛋白质，实际上在所有已被研究的物种中，其合成都是普遍存在的。在本研究中，我们使用流式细胞术研究了年龄和炎症对人外周血单个核细胞中Hsp27诱导的影响。66名健康对照受试者或患有炎症的患者参与了该研究。在热休克（HS）和非HS条件下，产生Hsp27的淋巴细胞百分比以及淋巴细胞和单核细胞中Hsp27的强度均受到年龄的负面影响。与淋巴细胞相比，单核细胞的基础水平以及HS后Hsp27的产生水平更高。此外，我们发现HS导致淋巴细胞中Hsp27水平有小幅但显著的增加，而单核细胞中Hsp27水平则显著下降。总之，本文给出的结果提供了证据，支持Hsp27水平与年龄相关的下降，而这种下降在炎症存在时消失。观察到循环中CRP、IL-6和TNF-α水平与各种Hsp27测定值之间存在几种关系，表明细胞因子能够影响Hsp27的产生。
