大肠杆菌中DNA聚合酶IV过量产生导致的突变体表型：滞后链上的优先诱变。
Mutator phenotype resulting from DNA polymerase IV overproduction in Escherichia coli: preferential mutagenesis on the lagging strand.
作者信息
Kuban Wojciech, Banach-Orlowska Magdalena, Bialoskorska Malgorzata, Lipowska Aleksandra, Schaaper Roel M, Jonczyk Piotr, Fijalkowska Iwona J
机构信息
Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5A, 02106 Warsaw, Poland.
出版信息
J Bacteriol. 2005 Oct;187(19):6862-6. doi: 10.1128/JB.187.19.6862-6866.2005.
Abstract
We investigated the mutator effect resulting from overproduction of Escherichia coli DNA polymerase IV. Using lac mutational targets in the two possible orientations on the chromosome, we observed preferential mutagenesis during lagging strand synthesis. The mutator activity likely results from extension of mismatches produced by polymerase III holoenzyme.
摘要
我们研究了大肠杆菌DNA聚合酶IV过量产生所导致的诱变效应。利用染色体上两种可能方向的乳糖突变靶点，我们观察到在滞后链合成过程中存在优先诱变现象。这种诱变活性可能是由聚合酶III全酶产生的错配延伸所致。
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本文引用的文献
1
DNA polymerase II as a fidelity factor in chromosomal DNA synthesis in Escherichia coli.DNA聚合酶II作为大肠杆菌染色体DNA合成中的保真因子。
Mol Microbiol. 2005 Oct;58(1):61-70. doi: 10.1111/j.1365-2958.2005.04805.x.
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Role of Escherichia coli DNA polymerase IV in in vivo replication fidelity.大肠杆菌DNA聚合酶IV在体内复制保真度中的作用。
J Bacteriol. 2004 Jul;186(14):4802-7. doi: 10.1128/JB.186.14.4802-4807.2004.
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Effect of deletion of SOS-induced polymerases, pol II, IV, and V, on spontaneous mutagenesis in Escherichia coli mutD5.SOS诱导的聚合酶II、IV和V缺失对大肠杆菌mutD5自发诱变的影响。
Environ Mol Mutagen. 2004;43(4):226-34. doi: 10.1002/em.20019.
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Polymerases leave fingerprints: analysis of the mutational spectrum in Escherichia coli rpoB to assess the role of polymerase IV in spontaneous mutation.聚合酶会留下印记：分析大肠杆菌rpoB中的突变谱以评估聚合酶IV在自发突变中的作用。
J Bacteriol. 2004 May;186(9):2900-5. doi: 10.1128/JB.186.9.2900-2905.2004.
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Chromosomal replicases as asymmetric dimers: studies of subunit arrangement and functional consequences.作为不对称二聚体的染色体复制酶：亚基排列及其功能后果的研究
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