Chung Hsiao-Hang, Hsieh Ming-Ju, Hsieh Yih-Shou, Chen Pei-Ni, Ko Chung-Po, Yu Nuo-Yi, Lin Chiao-Wen, Yang Shun-Fa
Department of Horticulture, National Ilan University, Yilan 260, Taiwan.
Oral Cancer Research Center, Changhua Christian Hospital, Changhua 500, Taiwan.
Pharmaceuticals (Basel). 2021 Nov 19;14(11):1183. doi: 10.3390/ph14111183.
Glioblastoma multiforme (GBM) is one of the most aggressive and common types of brain tumor. Due to its high proliferation ability, a high lethality rate has been observed with this malignant glial tumor. L. () is currently known to have anti-inflammatory and anti-carcinogenesis effects. However, few studies have examined the mechanisms of the leaf extracts of (TCE) on GBM cells. In the current study, we demonstrated that TCE can significantly inhibit the migration and invasion capabilities of GBM cell lines without showing biotoxic effects. Matrix metalloproteinases-2 (MMP-2) activity and protein expression were attenuated by reducing the p38 phosphorylation involved in the mitogen-activated protein kinase (MAPK) pathway. By treating with TCE and/or p38 inhibitor (SB203580), we confirmed that p38 MAPK is involved in the inhibition of cell migration. In conclusion, our results demonstrated that TCE inhibits human GBM cell migration and MMP-2 expression by regulating the p38 pathway. These results reveal that TCE contains potent therapeutic compounds which could be applied for treating GBM brain tumors.
多形性胶质母细胞瘤（GBM）是最具侵袭性且常见的脑肿瘤类型之一。由于其高增殖能力，这种恶性胶质肿瘤具有较高的致死率。目前已知L.（）具有抗炎和抗癌作用。然而，很少有研究探讨（TCE）叶提取物对GBM细胞的作用机制。在本研究中，我们证明TCE可显著抑制GBM细胞系的迁移和侵袭能力，且未表现出生物毒性作用。基质金属蛋白酶-2（MMP-2）的活性和蛋白表达通过减少丝裂原活化蛋白激酶（MAPK）途径中涉及的p38磷酸化而减弱。通过用TCE和/或p38抑制剂（SB203580）处理，我们证实p38 MAPK参与细胞迁移的抑制。总之，我们的结果表明TCE通过调节p38途径抑制人GBM细胞迁移和MMP-2表达。这些结果揭示TCE含有可用于治疗GBM脑肿瘤的有效治疗化合物。
