Moreira Emilia A M, Fagundes Regina L M, Filho Danilo Wilhelm, Neves Daniela, Sell Fabíola, Bellisle France, Kupek Emil
Departamento de Nutrição, Centro de Ciências da Saúde, Universidade Federal de Santa Catarina, Campus Universitário, Trindade, 88.040-970 Florianópolis, Brazil.
Clin Nutr. 2005 Dec;24(6):1038-46. doi: 10.1016/j.clnu.2005.08.005. Epub 2005 Sep 22.
Evaluate the influence of tomato powder in diets differing in energy level on antioxidant status in blood and liver of rats.
Twenty-four adult male Wistar rats weighing 150-180 g were placed four groups (n=6). For 28 days, animals were fed a diet that was either hyper energetic or hypo energetic. Some diets were supplemented with tomato powder. Liver and blood were collected for analysis of antioxidant enzymes, non-enzymatic antioxidants, thiobarbituric acid reactive species, ubiquinol 9, alpha-tocopherol, lycopene and beta-carotene. Data were analysed by two-way ANOVA.
Food intake and thiobarbituric acid-reactive substances contents in liver and plasma were significantly decreased by tomato powder at both energy levels. After tomato powder supplementation, the hepatic levels of ubiquinol 9, alpha-tocopherol, lycopene and beta-carotene were significantly enhanced. In plasma, only the contents of lycopene and beta-carotene were enhanced. The erythrocytic and hepatic activities of catalase were lower, while those of glutathione peroxidase were higher after the ingestion of tomato powder. Total and reduced glutathione contents in liver showed lower levels in cafeteria-fed rats compared to the hypo energetic diet.
The data suggest that the lycopene and beta-carotene component in the tomato power supplement might be beneficial for the prevention of oxidative damage in rats fed both types of energetic diets.
评估不同能量水平饮食中番茄粉对大鼠血液和肝脏抗氧化状态的影响。
将24只体重150 - 180克的成年雄性Wistar大鼠分为四组（每组n = 6）。持续28天，给动物喂食高能或低能饮食。部分饮食添加了番茄粉。采集肝脏和血液，用于分析抗氧化酶、非酶抗氧化剂、硫代巴比妥酸反应性物质、泛醇9、α-生育酚、番茄红素和β-胡萝卜素。数据采用双向方差分析。
在两种能量水平下，番茄粉均显著降低了肝脏和血浆中的食物摄入量以及硫代巴比妥酸反应性物质含量。补充番茄粉后，肝脏中泛醇9、α-生育酚、番茄红素和β-胡萝卜素水平显著提高。在血浆中，仅番茄红素和β-胡萝卜素含量增加。摄入番茄粉后，红细胞和肝脏中的过氧化氢酶活性降低，而谷胱甘肽过氧化物酶活性升高。与低能饮食相比，自由选择饮食的大鼠肝脏中总谷胱甘肽和还原型谷胱甘肽含量较低。
数据表明，番茄粉补充剂中的番茄红素和β-胡萝卜素成分可能有助于预防两种能量水平饮食喂养的大鼠的氧化损伤。
