Department of Zoology, Faculty of Science, Assiut University, Assiut, Egypt.
Fish Physiol Biochem. 2011 Mar;37(1):71-84. doi: 10.1007/s10695-010-9418-3. Epub 2010 Jul 13.
The present study investigates the potential protective effects of tomato paste (9 mg/kg-lycopene) in comparison with vitamin E (50 mg/kg) against the impacts of cadmium (Cd) toxicity (4.64 mg/l: ¼ of 96 h LC50) on fishes Cd exposed for 15 and 30 days. Cd impacts were evaluated in terms of biological, haematological and biochemical characteristics. Cd significantly induced free radicals in serum and liver. The activities of aspartate aminotransferase and alanine aminotransferase in serum were significantly increased due to Cd. Treatment with Cd caused a significant increase in Lipid peroxidation and DNA fragmentation in liver tissue and serum glucose and total lipid. On the other hand, Cd significantly led to decline in serum total protein, blood haemoglobin, red blood cell count, haematocrit value, mean corpuscular volume, mean corpuscular haemoglobin and mean corpuscular haemoglobin concentration. Dietary supplementation with vitamin E and/or tomato paste to Cd-exposed fish declined significantly the increased lipid peroxidation and DNA fragmentation in liver tissue and the increased aspartate aminotransferase, alanine aminotransferase, glucose and total lipid in serum to the normal condition. This supplementation also significantly increased the declined serum total protein, blood haemoglobin, red blood cell count, haematocrit value, mean corpuscular volume, mean corpuscular haemoglobin and mean corpuscular haemoglobin concentration to the normal state. Cd impacts and tomato paste/or vitamin E supplementations did not reflected on the condition factor of the fish. These findings demonstrated the beneficial diet supplementation of tomato paste phytonutrients and vitamin E in counteracting the harmful effects of Cd on the characters investigated.
本研究探讨了番茄酱（9mg/kg-番茄红素）与维生素 E（50mg/kg）在对抗鱼类暴露于镉（Cd）毒性（4.64mg/l：96hLC50 的四分之一）15 和 30 天后的潜在保护作用。Cd 的影响是通过生物、血液学和生化特征来评估的。Cd 显著诱导了血清和肝脏中的自由基。由于 Cd，血清中天冬氨酸氨基转移酶和丙氨酸氨基转移酶的活性显著增加。Cd 处理导致肝组织和血清中脂质过氧化和 DNA 片段化以及血清葡萄糖和总脂质显著增加。另一方面，Cd 显著导致血清总蛋白、血红蛋白、红细胞计数、血细胞比容、平均红细胞体积、平均红细胞血红蛋白和平均红细胞血红蛋白浓度下降。用维生素 E 和/或番茄酱补充饲料可显著降低肝组织中脂质过氧化和 DNA 片段化的增加，以及血清中天冬氨酸氨基转移酶、丙氨酸氨基转移酶、葡萄糖和总脂质的增加，使其恢复正常。这种补充还显著增加了血清总蛋白、血红蛋白、红细胞计数、血细胞比容、平均红细胞体积、平均红细胞血红蛋白和平均红细胞血红蛋白浓度的下降，使其恢复正常状态。Cd 的影响以及番茄酱/或维生素 E 的补充对鱼类的状况系数没有影响。这些发现表明，番茄红素植物营养素和维生素 E 的有益饮食补充可以对抗 Cd 对所研究特性的有害影响。
