Hamada J, Takeichi N, Okada F, Ren J, Li X, Hosokawa M, Kobayashi H
Laboratory of Pathology, Hokkaido University School of Medicine, Sapporo.
Jpn J Cancer Res. 1992 May;83(5):483-90. doi: 10.1111/j.1349-7006.1992.tb01953.x.
Tumor progression is the process by which tumor cells acquire more malignant properties, such as invasiveness and metastasis, during tumor development. To elucidate mechanisms of tumor progression, we examined the role of interactions between the tumor and its host by using a cloned cell line, ER-1, which was derived from a rat mammary carcinoma. ER-1 is weakly tumorigenic and non-metastatic when s.c. injected into syngeneic hosts in single cell suspension. However, ER-1 cells show a high incidence of lethal growth when s.c. implanted (5 x 10(2) cells), being attached to a 10 x 5 x 1 mm polystyrene plate. Tumor cell lines (PLT) obtained from tumors which had arisen from the plate-attached ER-1 cells no longer required plates for their growth in normal hosts, and had acquired metastatic ability to the lungs. The malignant phenotypes of PLT were stable under a usual culture condition for at least 6 months. Furthermore, the incidence of tumor development increased when small numbers of ER-1 cells were injected onto plates (or at their periphery) which had previously been implanted s.c. without tumor cells. The tumorigenicity of ER-1 cells increased after they were cocultivated for more than 30 days with host reactive cells obtained from the tissues surrounding the plates. These results suggest that host cells reactive to the foreign body (plastic plate) may not only promote the local growth of ER-1 cells but also convert them into much more malignant tumors.
肿瘤进展是指肿瘤细胞在肿瘤发展过程中获得更多恶性特性（如侵袭性和转移性）的过程。为了阐明肿瘤进展的机制，我们使用一种克隆细胞系ER-1来研究肿瘤与其宿主之间相互作用的作用，该细胞系源自大鼠乳腺癌。当以单细胞悬液皮下注射到同基因宿主中时，ER-1的致瘤性较弱且无转移性。然而，当将ER-1细胞（5×10²个细胞）附着于一块10×5×1毫米的聚苯乙烯板上进行皮下植入时，它们显示出高致死性生长发生率。从附着于板上的ER-1细胞产生的肿瘤中获得的肿瘤细胞系（PLT）在正常宿主中生长时不再需要板，并且获得了转移至肺部的能力。在通常的培养条件下，PLT的恶性表型至少6个月保持稳定。此外，当将少量ER-1细胞注射到先前已皮下植入但无肿瘤细胞的板上（或其周边）时，肿瘤发生的发生率增加。在与从板周围组织获得的宿主反应性细胞共培养超过30天后，ER-1细胞的致瘤性增加。这些结果表明，对异物（塑料板）有反应的宿主细胞不仅可能促进ER-1细胞的局部生长，还可能将它们转化为恶性程度更高的肿瘤。
