Hanani M
Laboratory of Experimental Surgery, Hadassah University Hospital, Mt. Scopus, Jerusalem, Israel.
J Auton Nerv Syst. 1992 May 1;38(2):77-84. doi: 10.1016/0165-1838(92)90228-9.
A method is described here for the vital staining of the nerve plexuses of the gastrointestinal tract with the fluorescent dye 4-(4-diethylaminostyryl)-methylpyridinium iodide (4-Di-2-ASP). This dye stained nerve fibers in the myenteric and submucosal plexuses and in the intramural ganglia of the gallbladder. In addition to staining the ganglia, the dye clearly revealed the secondary and the fine tertiary fibers of the myenteric plexus. In the submucosal plexus thin fibers innervating the blood vessels were also stained. This method proved to be effective in tissues that are relatively thick and in which the visualization of the nerves is quite difficult without staining--the myenteric plexus of rat small intestine, guinea-pig gallbladder and human large intestine. The staining did not fade within at least 6-8 h, or after long exposure to bright light. Intracellular recordings from myenteric neurons in the guinea pig ileum showed that cellular properties were not significantly altered after incubation in the dye solution. 4-Di-2-ASP thus appears to be suitable for rapid vital staining of nerves in whole mount from many parts of the gastrointestinal tract.
本文描述了一种用荧光染料4-(4-二乙氨基苯乙烯基)-甲基碘化吡啶(4-Di-2-ASP)对胃肠道神经丛进行活体染色的方法。这种染料可对肌间神经丛、黏膜下神经丛以及胆囊壁内神经节中的神经纤维进行染色。除了对神经节进行染色外，该染料还能清晰地显示肌间神经丛的二级和三级细纤维。在黏膜下神经丛中，支配血管的细纤维也被染色。该方法在相对较厚的组织中被证明是有效的，在这些组织中，如果不进行染色，神经的可视化相当困难，如大鼠小肠的肌间神经丛、豚鼠胆囊和人类大肠。染色至少在6 - 8小时内或长时间暴露于强光下都不会褪色。对豚鼠回肠肌间神经元进行的细胞内记录显示，在染料溶液中孵育后，细胞特性没有明显改变。因此，4-Di-2-ASP似乎适用于对胃肠道许多部位的神经进行快速活体全层染色。
