Liu Jikun, Sun Xuefei, Lee Milton L
Department of Chemistry and Biochemistry, Brigham Young University, Provo, Utah 84602-5700, USA.
Anal Chem. 2007 Mar 1;79(5):1926-31. doi: 10.1021/ac0617621. Epub 2007 Jan 24.
A poly(ethylene glycol)-functionalized acrylic copolymer was developed for fabrication of microfluidic devices that are resistant to protein and peptide adsorption. Planar microcapillary electrophoresis (microCE) devices were fabricated from this copolymer with the typical cross pattern to facilitate sample introduction. In contrast to most methods used to fabricate polymeric microchips, the photopolymerization-based method used with the copolymer reported in this work was of the soft lithography type, and both patterning and bonding could be completed within 10 min. In a finished microdevice, the cover plate and patterned substrate were bonded together through strong covalent bonds. Additionally, because of the resistance of the copolymer to adsorption, fabricated microfluidic devices could be used without surface modification to separate proteins and peptides. Separations of fluorescein isothiocyanate-labeled protein and peptide samples were accomplished using these new polymeric microCE microchips. Separation efficiencies as high as 4.7 x 10(4) plates were obtained in less than 40 s with a 3.5-cm separation channel, yielding peptide and protein peaks that were symmetrical.
开发了一种聚乙二醇功能化的丙烯酸共聚物，用于制造抗蛋白质和肽吸附的微流控装置。用这种共聚物制造了具有典型交叉图案的平面微毛细管电泳（microCE）装置，以方便样品引入。与大多数用于制造聚合物微芯片的方法不同，本文报道的与该共聚物一起使用的基于光聚合的方法属于软光刻类型，图案化和键合都可以在10分钟内完成。在制成的微器件中，盖板和图案化的基板通过强共价键结合在一起。此外，由于该共聚物具有抗吸附性，制造的微流控装置无需表面改性即可用于分离蛋白质和肽。使用这些新型聚合物microCE微芯片完成了异硫氰酸荧光素标记的蛋白质和肽样品的分离。在不到40秒的时间内，使用3.5厘米的分离通道获得了高达4.7×10⁴ 塔板数的分离效率，产生了对称的肽和蛋白质峰。
