Sau-PCR，一种用于微生物基因指纹分析的新型扩增技术。
Sau-PCR, a novel amplification technique for genetic fingerprinting of microorganisms.
作者信息
Corich Viviana, Mattiazzi Alessandro, Soldati Elisa, Carraro Angela, Giacomini Alessio
机构信息
Dipartimento di Biotecnologie Agrarie, Università di Padova, viale dell'Università 16, I-35020 Legnaro, Padova, Italy.
出版信息
Appl Environ Microbiol. 2005 Oct;71(10):6401-6. doi: 10.1128/AEM.71.10.6401-6406.2005.
Abstract
The proposed technique is based on the digestion of genomic DNA with the restriction endonuclease Sau3AI and subsequent amplification with primers whose core sequence is based on the Sau3AI recognition site. The method was tested on strains of lactic acid bacteria but could be proposed for virtually any culturable organism from which DNA can be extracted.
摘要
所提出的技术基于用限制性内切酶Sau3AI消化基因组DNA，随后用其核心序列基于Sau3AI识别位点的引物进行扩增。该方法已在乳酸菌菌株上进行了测试，但实际上可用于任何能够提取DNA的可培养生物体。
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