溃疡分枝杆菌中多态性可变数目串联重复序列位点的比较核苷酸序列分析
Comparative nucleotide sequence analysis of polymorphic variable-number tandem-repeat Loci in Mycobacterium ulcerans.
作者信息
Ablordey Anthony, Hilty Markus, Stragier Pieter, Swings Jean, Portaels Françoise
机构信息
Mycobacteriology Unit, Prince Leopold Institute of Tropical Medicine, B-2000 Antwerp, Belgium.
出版信息
J Clin Microbiol. 2005 Oct;43(10):5281-4. doi: 10.1128/JCM.43.10.5281-5284.2005.
Abstract
We analyzed a set of variable-number tandem-repeat (VNTR) loci to assess their nucleotide sequence diversity in isolates of three Mycobacterium ulcerans genotypes. Sequence variants in two loci resulted in intraspecies resolution of Southeast Asian and Asian genotypes in contrast to a homogenous sequence composition among African isolates. Nucleotide sequence polymorphism in repeat units can enhance discrimination of VNTR loci.
摘要
我们分析了一组可变数目串联重复序列（VNTR）位点，以评估三种溃疡分枝杆菌基因型分离株中的核苷酸序列多样性。与非洲分离株中均一的序列组成不同，两个位点的序列变异导致东南亚和亚洲基因型在种内得以区分。重复单元中的核苷酸序列多态性可增强VNTR位点的鉴别能力。
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