环丝氨酸-头孢西丁-果糖琼脂和L-脯氨酸氨基肽酶（PRO试纸）在艰难梭菌快速鉴定中的应用
Use of cycloserine-cefoxitin-fructose agar and L-proline-aminopeptidase (PRO Discs) in the rapid identification of Clostridium difficile.
作者信息
Fedorko D P, Williams E C
机构信息
Clinical Pathology Department, Warren G. Magnuson, Clinical Center, National Institutes of Health, Bethesda, Maryland 20892, USA.
出版信息
J Clin Microbiol. 1997 May;35(5):1258-9. doi: 10.1128/jcm.35.5.1258-1259.1997.
Abstract
The PRO Disc (Carr-Scarborough Microbiologicals, Inc., Decatur, Ga.) can be used to screen for L-proline-aminopeptidase produced by Clostridium difficile grown on cycloserine-cefoxitin-fructose agar (CCFA). Fifty stored isolates of C. difficile (48 toxin-positive and 2 toxin-negative isolates) and 47 fresh C. difficile isolates (39 toxin-positive and 8 toxin-negative isolates) were all PRO Disc positive. Other Clostridium species that were PRO Disc positive could be differentiated from C. difficile by failure to grow on CCFA, different colonial morphology on CCFA, or morphology upon Gram staining.
摘要
PRO检测盘（卡尔-斯卡伯勒微生物公司，佐治亚州迪凯特）可用于筛选艰难梭菌在环丝氨酸-头孢西丁-果糖琼脂（CCFA）上生长时产生的L-脯氨酸氨基肽酶。五十株保存的艰难梭菌分离株（48株毒素阳性和2株毒素阴性分离株）以及47株新鲜的艰难梭菌分离株（39株毒素阳性和8株毒素阴性分离株）均为PRO检测盘阳性。其他PRO检测盘阳性的梭菌属菌种可通过在CCFA上不生长、在CCFA上不同的菌落形态或革兰氏染色后的形态与艰难梭菌区分开来。
相似文献
1
Use of cycloserine-cefoxitin-fructose agar and L-proline-aminopeptidase (PRO Discs) in the rapid identification of Clostridium difficile.环丝氨酸-头孢西丁-果糖琼脂和L-脯氨酸氨基肽酶（PRO试纸）在艰难梭菌快速鉴定中的应用
J Clin Microbiol. 1997 May;35(5):1258-9. doi: 10.1128/jcm.35.5.1258-1259.1997.
2
Evaluation of cycloserine-cefoxitin fructose agar (CCFA), CCFA with horse blood and taurocholate, and cycloserine-cefoxitin mannitol broth with taurocholate and lysozyme for recovery of Clostridium difficile isolates from fecal samples.评估环丝氨酸头孢西丁果糖琼脂（CCFA）、含马血和牛胆酸钠的 CCFA 以及含牛胆酸钠和溶菌酶的环丝氨酸头孢西丁甘露醇肉汤，用于从粪便样本中回收艰难梭菌分离株。
J Clin Microbiol. 2013 Sep;51(9):3094-6. doi: 10.1128/JCM.00879-13. Epub 2013 Jun 26.
3
Evaluation of cycloserine-cefoxitin-fructose agar and cycloserine-cefoxitin-fructose broth for recovery of Clostridium difficile from environmental sites.评估环丝氨酸-头孢西丁-果糖琼脂和环丝氨酸-头孢西丁-果糖肉汤用于从环境场所中分离艰难梭菌的效果。
J Clin Microbiol. 1991 Nov;29(11):2633-5. doi: 10.1128/jcm.29.11.2633-2635.1991.
4
Improved recovery of Clostridium difficile spores with the incorporation of synthetic taurocholate in cycloserine-cefoxitin-fructose agar (CCFA).在环丝氨酸头孢西丁果糖琼脂（CCFA）中加入合成牛磺胆酸钠可提高艰难梭菌孢子的回收率。
Pathology. 2012 Jun;44(4):354-6. doi: 10.1097/PAT.0b013e328353a235.
5
Comparison of cycloserine-cefoxitin-fructose agar (CCFA) and taurocholate-CCFA for recovery of Clostridium difficile during surveillance of hospitalized patients.在住院患者监测期间，比较环丝氨酸-头孢西丁-果糖琼脂（CCFA）和牛磺胆酸盐-CCFA用于艰难梭菌分离培养的效果。
Diagn Microbiol Infect Dis. 1997 Sep;29(1):1-4. doi: 10.1016/s0732-8893(97)00113-2.
6
Comparison of five cultural procedures for isolation of Clostridium difficile from stools.从粪便中分离艰难梭菌的五种培养方法的比较。
J Clin Microbiol. 1992 Feb;30(2):514-6. doi: 10.1128/jcm.30.2.514-516.1992.
7
Isolation of Clostridium difficile from faecal specimens--a comparison of chromID C. difficile agar and cycloserine-cefoxitin-fructose agar.从粪便标本中分离艰难梭菌——显色艰难梭菌琼脂与环丝氨酸头孢西丁果糖琼脂的比较。
J Med Microbiol. 2013 Sep;62(Pt 9):1423-1427. doi: 10.1099/jmm.0.056515-0. Epub 2013 Apr 11.
8
Proline-aminopeptidase test for rapid screening of Clostridium difficile.用于快速筛查艰难梭菌的脯氨酸氨肽酶试验
J Clin Microbiol. 1997 Nov;35(11):3007. doi: 10.1128/jcm.35.11.3007-3007.1997.
9
Comparison of culture based methods for the isolation of Clostridium difficile from stool samples in a research setting.在研究环境中，比较基于培养的方法从粪便样本中分离艰难梭菌。
Anaerobe. 2014 Aug;28:226-9. doi: 10.1016/j.anaerobe.2014.07.003. Epub 2014 Jul 16.
10
Development and Validation of a New Protocol for Detecting and Recovering Clostridium difficile from Meat Samples.一种用于从肉类样本中检测和分离艰难梭菌的新方案的开发与验证
J Food Prot. 2018 Apr;81(4):561-568. doi: 10.4315/0362-028X.JFP-17-354.
引用本文的文献
1
Clostridial Diseases of Horses: A Review.马的梭菌病：综述
Vaccines (Basel). 2022 Feb 17;10(2):318. doi: 10.3390/vaccines10020318.
2
Clostridioides (Clostridium) difficile in neonatal foals and mares at a referral hospital.在一家转诊医院的新生驹和母马中艰难梭菌（梭状芽孢杆菌属）。
J Vet Intern Med. 2021 Mar;35(2):1140-1146. doi: 10.1111/jvim.16094. Epub 2021 Mar 3.
3
Prevalence of Isolated from Beef and Chicken Meat Products in Turkey.土耳其牛肉和鸡肉制品中分离出的[病原体名称未给出]的患病率。 （注：原文中“Isolated from”前缺少具体病原体名称，翻译时按此处理。）
Korean J Food Sci Anim Resour. 2018 Sep;38(4):759-767. doi: 10.5851/kosfa.2018.e14. Epub 2018 Sep 30.
4
Pathogenicity locus determinants and toxinotyping of isolates recovered from Iranian patients.从伊朗患者中分离出的菌株的致病位点决定因素及毒素分型
New Microbes New Infect. 2018 Aug 2;25:52-57. doi: 10.1016/j.nmni.2018.07.003. eCollection 2018 Sep.
5
Effect of the Synthetic Bile Salt Analog CamSA on the Hamster Model of Clostridium difficile Infection.合成胆盐水解物 CamSA 对艰难梭菌感染仓鼠模型的影响。
Antimicrob Agents Chemother. 2018 Sep 24;62(10). doi: 10.1128/AAC.02251-17. Print 2018 Oct.
6
Antimicrobial susceptibility and ribotypes of Clostridium difficile isolates from a Phase 2 clinical trial of ridinilazole (SMT19969) and vancomycin.利奈唑胺（SMT19969）和万古霉素治疗艰难梭菌感染的 2 期临床试验中艰难梭菌分离株的药敏性和核糖体分型。
J Antimicrob Chemother. 2018 Aug 1;73(8):2078-2084. doi: 10.1093/jac/dky135.
7
Toxigenic in retail packed chicken meat and broiler flocks in northeastern Iran.伊朗东北部零售包装鸡肉和肉鸡群中的产毒情况。
Iran J Vet Res. 2017 Fall;18(4):271-274.
8
Role of Proline in Pathogen and Host Interactions.脯氨酸在病原体与宿主相互作用中的作用。
Antioxid Redox Signal. 2019 Feb 1;30(4):683-709. doi: 10.1089/ars.2017.7335. Epub 2018 Feb 2.
9
Biosecurity practices and causes of enteritis on Ontario meat rabbit farms.安大略省肉兔养殖场的生物安全措施及肠炎病因
Can Vet J. 2017 Jun;58(6):571-578.
10
Isolation of and molecular detection of binary and A/B toxins in faeces of dogs.犬粪便中二元毒素和A/B毒素的分离及分子检测
Iran J Vet Res. 2016 Fall;17(4):273-276.
本文引用的文献
1
Role of culture and toxin detection in laboratory testing for diagnosis of Clostridium difficile-associated diarrhea.培养和毒素检测在艰难梭菌相关性腹泻诊断实验室检测中的作用
Eur J Clin Microbiol Infect Dis. 1996 Apr;15(4):330-6. doi: 10.1007/BF01695667.
2
Laboratory detection of Clostridium difficile. A comparison of media and incubation systems.艰难梭菌的实验室检测。培养基与培养系统的比较。
Am J Clin Pathol. 1995 Jan;103(1):52-6. doi: 10.1093/ajcp/103.1.52.
3
Rapid identification of Clostridium difficile by toxin detection.通过毒素检测快速鉴定艰难梭菌。
J Clin Microbiol. 1982 Mar;15(3):465-7. doi: 10.1128/jcm.15.3.465-467.1982.
4
Simple method for isolation and presumptive identification of Clostridium difficile.艰难梭菌分离与初步鉴定的简易方法
Zentralbl Bakteriol Mikrobiol Hyg A Med Mikrobiol Infekt Parasitol. 1981;250(1-2):142-6.
5
Simplified procedure for the routine isolation of Clostridium difficile from faeces.从粪便中常规分离艰难梭菌的简化程序。
J Clin Pathol. 1981 Oct;34(10):1124-7. doi: 10.1136/jcp.34.10.1124.
6
Clostridium difficile: its disease and toxins.艰难梭菌：其所致疾病与毒素
Clin Microbiol Rev. 1988 Jan;1(1):1-18. doi: 10.1128/CMR.1.1.1.
7
Results of a prospective, 18-month clinical evaluation of culture, cytotoxin testing, and culturette brand (CDT) latex testing in the diagnosis of Clostridium difficile-associated diarrhea.一项关于培养、细胞毒素检测和拭子品牌（CDT）乳胶检测用于艰难梭菌相关性腹泻诊断的前瞻性18个月临床评估结果。
Diagn Microbiol Infect Dis. 1988 Jun;10(2):85-91. doi: 10.1016/0732-8893(88)90045-4.
Zotero 插件