Marin Lauro Juliano, Cunha Aldo Albuquerque, Aquino Victor Hugo, Figueiredo Luiz Tadeu Moraes
Unidade Multidepartamental de Pesquisa em Virologia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirao Preto, SP, Brazil.
Rev Soc Bras Med Trop. 2004 Mar-Apr;37(2):158-64. doi: 10.1590/s0037-86822004000200009. Epub 2004 Apr 13.
The purpose of this work was to test a cytomegalovirus qualitative PCR and a semi-quantitative PCR on the determination of CMV load in leukocytes of bone marrow and kidney transplanted (RT) patients. Thirty three BMT and 35 RT patients participated of the study. The DNA was subjected to a qualitative PCR using primers that amplify part of CMV gB gene. CMV load of positive samples was determined by a semi-quantitative PCR using quantified plasmids inserted with part of the gB gene of CMV as controls. The sensitivity of the test was determined to be 867 plasmid copies/ microg DNA. CMV loads between 2,118 and 72,443 copies/ microg DNA were observed in 12.1% BMT recipients and between 1,246 and 58,613 copies/ microg DNA in 22.9% RT recipients. Further studies are necessary to confirm the usefulness of this CMV semi-quantitative PCR in transplanted patients.
这项工作的目的是测试一种巨细胞病毒定性PCR和一种半定量PCR在测定骨髓和肾移植（RT）患者白细胞中巨细胞病毒载量方面的效果。33名骨髓移植患者和35名肾移植患者参与了该研究。使用能扩增巨细胞病毒gB基因部分片段的引物对DNA进行定性PCR。阳性样本的巨细胞病毒载量通过使用插入了巨细胞病毒gB基因部分片段的定量质粒作为对照的半定量PCR来测定。该检测的灵敏度确定为867个质粒拷贝/微克DNA。在12.1%的骨髓移植受者中观察到巨细胞病毒载量在2,118至72,443拷贝/微克DNA之间，在22.9%的肾移植受者中观察到巨细胞病毒载量在1,246至58,613拷贝/微克DNA之间。需要进一步研究以证实这种巨细胞病毒半定量PCR在移植患者中的实用性。
