Bonanno Jeffrey B, Almo Steven C, Bresnick Anne, Chance Mark R, Fiser Andras, Swaminathan S, Jiang J, Studier F William, Shapiro Lawrence, Lima Christopher D, Gaasterland Theresa M, Sali Andrej, Bain Kevin, Feil Ingeborg, Gao Xia, Lorimer Don, Ramos Aurora, Sauder J Michael, Wasserman Steven R, Emtage Spencer, D'Amico Kevin L, Burley Stephen K
Structural GenomiX, Inc., 10505 Roselle Street, San Diego, CA 92121, USA.
J Struct Funct Genomics. 2005;6(2-3):225-32. doi: 10.1007/s10969-005-6827-0.
Structural GenomiX, Inc. (SGX), four New York area institutions, and two University of California schools have formed the New York Structural GenomiX Research Consortium (NYSGXRC), an industrial/academic Research Consortium that exploits individual core competencies to support all aspects of the NIH-NIGMS funded Protein Structure Initiative (PSI), including protein family classification and target selection, generation of protein for biophysical analyses, sample preparation for structural studies, structure determination and analyses, and dissemination of results. At the end of the PSI Pilot Study Phase (PSI-1), the NYSGXRC will be capable of producing 100-200 experimentally determined protein structures annually. All Consortium activities can be scaled to increase production capacity significantly during the Production Phase of the PSI (PSI-2). The Consortium utilizes both centralized and de-centralized production teams with clearly defined deliverables and hand-off procedures that are supported by a web-based target/sample tracking system (SGX Laboratory Information Data Management System, LIMS, and NYSGXRC Internal Consortium Experimental Database, ICE-DB). Consortium management is provided by an Executive Committee, which is composed of the PI and all Co-PIs. Progress to date is tracked on a publicly available Consortium web site (http://www.nysgxrc.org) and all DNA/protein reagents and experimental protocols are distributed freely from the New York City Area institutions. In addition to meeting the requirements of the Pilot Study Phase and preparing for the Production Phase of the PSI, the NYSGXRC aims to develop modular technologies that are transferable to structural biology laboratories in both academe and industry. The NYSGXRC PI and Co-PIs intend the PSI to have a transforming effect on the disciplines of X-ray crystallography and NMR spectroscopy of biological macromolecules. Working with other PSI-funded Centers, the NYSGXRC seeks to create the structural biology laboratory of the future. Herein, we present an overview of the organization of the NYSGXRC and describe progress toward development of a high-throughput Gene-->Structure platform. An analysis of current and projected consortium metrics reflects progress to date and delineates opportunities for further technology development.
结构基因组学公司（SGX）、纽约地区的四家机构以及加利福尼亚大学的两所学院共同组建了纽约结构基因组学研究联盟（NYSGXRC），这是一个产学研研究联盟，利用各自的核心能力来支持美国国立卫生研究院国家综合医学科学研究所（NIH-NIGMS）资助的蛋白质结构计划（PSI）的各个方面，包括蛋白质家族分类和靶点选择、用于生物物理分析的蛋白质生成、结构研究的样品制备、结构测定与分析以及结果传播。在PSI试点研究阶段（PSI-1）结束时，NYSGXRC每年将能够产出100 - 200个通过实验确定的蛋白质结构。在PSI的生产阶段（PSI-2），联盟的所有活动都可以进行扩展，以显著提高生产能力。该联盟利用集中式和分散式生产团队，拥有明确界定的可交付成果和交接程序，并由基于网络的靶点/样品跟踪系统（SGX实验室信息数据管理系统，LIMS，以及NYSGXRC内部联盟实验数据库，ICE-DB）提供支持。联盟管理由一个执行委员会负责，该委员会由项目负责人（PI）和所有共同项目负责人（Co-PIs）组成。迄今为止的进展情况在一个公开的联盟网站（http://www.nysgxrc.org）上进行跟踪，所有DNA/蛋白质试剂和实验方案都在纽约市地区的机构免费分发。除了满足试点研究阶段的要求并为PSI的生产阶段做准备外，NYSGXRC旨在开发可转移到学术界和工业界结构生物学实验室的模块化技术。NYSGXRC的项目负责人和共同项目负责人希望PSI能对生物大分子的X射线晶体学和核磁共振光谱学学科产生变革性影响。与其他由PSI资助的中心合作，NYSGXRC致力于打造未来的结构生物学实验室。在此，我们概述了NYSGXRC的组织架构，并描述了高通量基因→结构平台的开发进展。对联盟当前和预计指标的分析反映了迄今为止的进展，并勾勒出进一步技术开发的机会。
