Bock Nicholas A, Nieman Brian J, Bishop Johnathan B, Mark Henkelman R
Department of Medical Biophysics, University of Toronto, Toronto, Canada.
Magn Reson Med. 2005 Nov;54(5):1311-6. doi: 10.1002/mrm.20683.
We developed a live high-field multiple-mouse magnetic resonance imaging method to increase the throughput of imaging studies involving large numbers of mice. Phantom experiments were performed in 7 shielded radiofrequency (RF) coils for concurrent imaging on a 7 Tesla MRI scanner outfitted with multiple transmit and receive channels to confirm uniform signal-to-noise ratio and minimal ghost artifacts across images from the different RF coils. Grid phantoms were used to measure image distortion in different positions in the coils. The brains of 7 live mice were imaged in 3D in the RF coil array, and a second array of 16 RF coils was used to 3D image the whole bodies of 16 fixed, contrast agent-perfused mice. The images of the 7 live mouse brains at 156 microm isotropic resolution and the 16 whole fixed mice at 100 microm isotropic resolution were of high quality and free of artifacts. We have thus shown that multiple-mouse MRI increases throughput for live and fixed mouse experiments by a factor equaling the number of RF coils in the scanner.
我们开发了一种实时高场多小鼠磁共振成像方法，以提高涉及大量小鼠的成像研究的通量。在配备多个发射和接收通道的7特斯拉MRI扫描仪上，在7个屏蔽射频（RF）线圈中进行了体模实验，用于同时成像，以确认不同RF线圈图像之间的信噪比均匀且鬼影伪影最小。使用网格体模测量线圈中不同位置的图像失真。在RF线圈阵列中对7只活小鼠的大脑进行了三维成像，并使用第二个由16个RF线圈组成的阵列对16只固定的、灌注了造影剂的小鼠的全身进行了三维成像。7只活小鼠大脑在156微米各向同性分辨率下的图像以及16只固定小鼠全身在100微米各向同性分辨率下的图像质量很高且无伪影。因此，我们已经表明，多小鼠MRI可使活小鼠和固定小鼠实验的通量提高到与扫描仪中RF线圈数量相等的倍数。
