Sharma Sandeep, Kaur Gurcharan
Neurochemistry and Neuroendocrinology Lab, Department of Biotechnology, Guru Nanak Dev University, Amritsar, Punjab 143005, India.
Brain Res Bull. 2005 Nov 30;67(6):482-91. doi: 10.1016/j.brainresbull.2005.07.015. Epub 2005 Aug 30.
The influence that dietary factors have on the nervous system and its susceptibility to disease, is an active area of biomedical research. Recent studies have shown that dietary restriction (DR) can have profound effect on brain function and vulnerability to injury and disease and can also enhance synaptic plasticity, which may increase the ability of brain to resist aging and restore function following injury. The dietary restriction may result in neuroprotection as suggested by marked reduction in neuronal cell death of the CA3 region of hippocampus after kainate administration in our study. We examined the effects of 3 months of DR (alternate day feeding regimen) on the antioxidants and antioxidant enzymes from different brain regions such as cerebral hemispheres, diencephalon, cerebellum and brain stem after kainate-induced excitotoxicity in adult male Wistar rats. The present study reports the beneficial effects of dietary restriction on different antioxidants and antioxidant enzymes against kainate-induced excitotoxicity in different brain regions of young adult male Wistar rats. The expression of stress response protein heat shock protein 70 (HSP 70) was also studied from discrete regions of rat brain under the same set of experimental conditions. DR significantly enhanced the expression of HSP 70 in kainic acid (KA)-treated rats, whereas KA treatment of ad libitum fed rats resulted in decreased HSP 70 expression. The DR was observed to exert neuroprotection by enhancing the expression of HSP 70 in kainic acid treated rats.
饮食因素对神经系统及其疾病易感性的影响，是生物医学研究的一个活跃领域。最近的研究表明，饮食限制（DR）可对脑功能以及对损伤和疾病的易感性产生深远影响，还可增强突触可塑性，这可能会提高大脑抵抗衰老以及损伤后恢复功能的能力。如我们的研究所表明，在给予红藻氨酸后，海马体CA3区神经元细胞死亡显著减少，这提示饮食限制可能具有神经保护作用。我们研究了在成年雄性Wistar大鼠中，红藻氨酸诱导兴奋性毒性后，3个月的饮食限制（隔日喂养方案）对大脑不同区域（如大脑半球、间脑、小脑和脑干）的抗氧化剂和抗氧化酶的影响。本研究报告了饮食限制对成年雄性Wistar大鼠不同脑区中红藻氨酸诱导的兴奋性毒性的不同抗氧化剂和抗氧化酶的有益作用。在同一组实验条件下，还从大鼠脑的离散区域研究了应激反应蛋白热休克蛋白70（HSP 70）的表达。饮食限制显著增强了经红藻氨酸（KA）处理的大鼠中HSP 70的表达，而对自由进食的大鼠进行KA处理则导致HSP 70表达降低。观察到饮食限制通过增强经红藻氨酸处理的大鼠中HSP 70的表达发挥神经保护作用。
