Centre for Land and Water Resource Management, G. B. Pant National Institute of Himalayan Environment, Kosi-Katarmal, Almora, 263643, Uttarakhand, India.
Department of Biotechnology, Graphic Era Deemed to be University, Bell Road, Clement Town, Dehra Dun, 248002, Uttarakhand, India.
Arch Microbiol. 2022 Apr 21;204(5):272. doi: 10.1007/s00203-022-02885-y.
A psychrotolerant bacterial strain of Pseudomonas sp. (P. palleroniana GBPI_508), isolated from the Indian Himalayan region, is studied for analyzing its potential for degrading bisphenol A (BPA). Response surface methodology using Box-Behnken design was used to statistically optimize the environmental factors during BPA degradation and the maximum degradation (97%) was obtained at optimum conditions of mineral salt media pH 9, experimental temperature 25 °C, an inoculum volume of 10% (v/v), and agitation speed 130 rpm at the BPA concentration 270 mg L. The Monod model was used for understanding bacterial degradation kinetics, and 37.5 mg half saturation coefficient (K) and 0.989 regression coefficient (R) were obtained. Besides, the utmost specific growth rate µmax was witnessed as 0.080 h with the GBPI_508 during BPA degradation. Metabolic intermediates detected in this study by GC-MS were identified as valeric acid, propionic acid, diglycolic acid, and phenol. The psychrotolerant bacterial strain of Pseudomonas sp. (P. palleroniana GBPI_508), isolated from the Indian Himalayan region has shown good potential for remediation of BPA at variable conditions.
从印度喜马拉雅地区分离得到的耐冷假单胞菌（P. palleroniana GBPI_508）菌株被研究用于分析其降解双酚 A（BPA）的潜力。使用 Box-Behnken 设计的响应面法对 BPA 降解过程中的环境因素进行了统计学优化，在最佳条件下（矿盐培养基 pH 9、实验温度 25°C、接种量 10%（v/v）和搅拌速度 130rpm），BPA 浓度为 270mg/L 时，最大降解率达到 97%。使用 Monod 模型来理解细菌降解动力学，得到了 37.5mg 的半饱和系数（K）和 0.989 的回归系数（R）。此外，在 BPA 降解过程中，GBPI_508 的最大比生长速率µmax为 0.080h。通过 GC-MS 检测到的本研究中的代谢中间产物被鉴定为戊酸、丙酸、二甘醇酸和苯酚。从印度喜马拉雅地区分离得到的耐冷假单胞菌（P. palleroniana GBPI_508）菌株在不同条件下对 BPA 的修复具有良好的潜力。
