Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand.
J Nat Med. 2012 Apr;66(2):279-83. doi: 10.1007/s11418-011-0582-2. Epub 2011 Aug 28.
Asiaticoside has been identified as the most active compound in Centella asiatica. In order to screen a large number of plant samples for the presence of asiaticoside, a rapid and simple technique is required that utilizes small quantities for test samples. In this study, an immunochromatographic strip test has been developed for the detection of asiaticoside in plant samples that uses a monoclonal antibody against asiaticoside. The limit of detection for the strip test was 12.5 μg/ml. Immunoassay using monoclonal antibodies could be useful for the determination of small quantities of asiaticoside in plant extracts.
积雪草苷已被鉴定为积雪草中的最有效化合物。为了筛选大量含有积雪草苷的植物样本，需要一种利用少量测试样本的快速简单技术。在这项研究中，开发了一种免疫层析条检测法，用于检测植物样本中的积雪草苷，该方法使用针对积雪草苷的单克隆抗体。条带检测的检测限为 12.5μg/ml。使用单克隆抗体的免疫测定法可能有助于测定植物提取物中的少量积雪草苷。
