Hashimoto K, Joseph F, Falster A U, Simmons W B, Nakamoto T
Department of Physiology, Louisiana State University Medical Center, New Orleans 70119.
Arch Oral Biol. 1992 Feb;37(2):105-9. doi: 10.1016/0003-9969(92)90005-s.
Dams were fed normal laboratory chow until delivery. At birth, the litters were combined, and eight pups were randomly assigned to each dam. Dams with the recombined litters were divided into two groups. Dams of group 1 were fed a 20% protein diet as a control; dams of group 2 were fed a 20% protein diet supplemented with caffeine (2 mg/100 g of the dam's weight). On day 22, the dams of group 2 were anaesthetized with ether. They were injected with 2 iu of oxytocin in order to collect milk. Blood was collected from pups and dams to determine its caffeine concentration. The first and second molars were removed from each pup's mandible and maxilla. Radiographs were taken of 10 randomly selected first or second molars from each group. Four randomly selected molars from each litter were placed in a specially designed chamber and bathed with a constant flow of acid solution to determine the amount of mineral dissolved from the enamel surfaces. The remaining non-acid exposed molars were pulverized in freezer mills. A small portion of this powder was then analysed for the total amount of minerals. No differences were found in the radiographic density of enamel between the groups. The amount of dissolved calcium, phosphorus and magnesium from enamel surfaces in the caffeine group was consistently greater than that of the non-caffeine group in the first molars, whereas, in the second molars, there was no difference between the groups.(ABSTRACT TRUNCATED AT 250 WORDS)
母鼠在分娩前一直喂食普通实验室饲料。幼崽出生时，将各窝幼崽合并，然后随机分配给每只母鼠八只幼崽。合并幼崽后的母鼠被分为两组。第1组母鼠喂食20%蛋白质饮食作为对照；第2组母鼠喂食添加咖啡因（2毫克/100克母鼠体重）的20%蛋白质饮食。在第22天，第2组母鼠用乙醚麻醉。为了收集乳汁，给它们注射了2国际单位的催产素。从幼崽和母鼠身上采集血液以测定咖啡因浓度。从每只幼崽的下颌骨和上颌骨中取出第一和第二磨牙。对每组中随机选择的10颗第一或第二磨牙进行X射线摄影。从每窝中随机选择四颗磨牙，放入一个专门设计的腔室中，用恒定流速的酸性溶液冲洗，以测定从牙釉质表面溶解的矿物质含量。其余未暴露于酸性溶液的磨牙在冷冻研磨机中磨碎。然后取一小部分这种粉末分析矿物质的总量。两组之间牙釉质的X射线密度没有差异。在第一磨牙中，咖啡因组从牙釉质表面溶解的钙、磷和镁的量始终大于非咖啡因组，而在第二磨牙中，两组之间没有差异。（摘要截断于250字）
