Falster A U, Hashimoto K, Nakamoto T, Simmons W B
Department of Geology and Geophysics, University of New Orleans, Lakefront, LA 70148.
Arch Oral Biol. 1992 Feb;37(2):111-8. doi: 10.1016/0003-9969(92)90006-t.
Samples of the first molars of offspring whose dams were fed a diet supplemented with caffeine were examined by scanning electron microscopy, X-ray diffractometry and electron microprobe analysis. Scanning microscopy of the enamel surface of the caffeine group revealed a consistently rougher surface than in the non-caffeine controls, both before and after acid exposure. X-ray diffraction analysis of the pulverized whole tooth in the caffeine group showed broader diffraction peaks for the lattice plane reflections (202) and (300), indicating smaller crystallites. Pure enamel samples of the caffeine group examined with a Gandolfi X-ray camera also revealed more diffuse diffraction lines than in the non-caffeine controls, further indicating smaller crystallites in the enamel. The calcium and phosphorus contents of the acid-exposed samples in both control and caffeine groups were lower than the non-acid exposed control and caffeine groups by electron microprobe analysis. After exposure to acid, the calcium and phosphorus contents of the outer surface of the enamel in the caffeine group were greatly reduced as compared to that of the non-caffeine controls. Thus various methods consistently indicate that caffeine ingestion during early growth affects the enamel surface of the first molars, resulting in impaired mineralization. Caffeine intake may therefore have a negative effect on amelogenesis and possibly increases susceptibility to dental caries.
对母鼠喂食添加咖啡因饮食的后代第一磨牙样本进行了扫描电子显微镜、X射线衍射和电子微探针分析。咖啡因组牙釉质表面的扫描显微镜检查显示，在酸暴露前后，其表面始终比非咖啡因对照组更粗糙。咖啡因组磨碎的全牙的X射线衍射分析显示，晶格平面反射（202）和（300）的衍射峰更宽，表明微晶更小。用甘道夫X射线相机检查的咖啡因组纯牙釉质样本也显示出比非咖啡因对照组更弥散的衍射线，进一步表明牙釉质中的微晶更小。通过电子微探针分析，对照组和咖啡因组酸暴露样本中的钙和磷含量均低于未酸暴露的对照组和咖啡因组。与非咖啡因对照组相比，咖啡因组牙釉质外表面在酸暴露后的钙和磷含量大大降低。因此，各种方法一致表明，早期生长期间摄入咖啡因会影响第一磨牙的牙釉质表面，导致矿化受损。因此，摄入咖啡因可能对釉质形成产生负面影响，并可能增加患龋齿的易感性。
