Meissner Peter E, Coulibaly Boubacar, Mandi Germain, Mansmann Ulrich, Witte Steffen, Schiek Wolfgang, Müller Olaf, Schirmer R Heiner, Mockenhaupt Frank P, Bienzle Ulrich
Department of Tropical Hygiene and Public Health, Ruprecht-Karls-University, Heidelberg, Germany.
Br J Haematol. 2005 Nov;131(3):395-9. doi: 10.1111/j.1365-2141.2005.05778.x.
Glucose-6-phosphate dehydrogenase (G6PD) deficient individuals are at increased risk of developing haemolysis following treatment with various antimalarial drugs. Reliable field tests for G6PD deficiency are thus needed in chemotherapy studies and their validity has to be assessed. In two phase II clinical trials on methylene blue (MB) antimalarial therapy in rural Burkina Faso, paediatric and adult participants were tested for G6PD deficiency. The results of a haemoglobin-adjusted nicotinamide adenine dinucleotide phosphate (NADPH) fluorescence test on paper (NFP test) were compared with polymerase chain reaction (PCR)-based G6PD genotyping also using blood samples on filter papers. This is the first study comparing sensitivity and specificity of the two methods. There was good agreement between the NFP test results and the PCR findings. The estimate of the sensitivity of the NFP test was 98.2% (95.8-99.6%) and the specificity was 97.1% (94.2-99.2%). In conclusion, the NFP assay is a reliable and inexpensive method for large-scale G6PD deficiency screening in rural West Africa.
葡萄糖-6-磷酸脱氢酶（G6PD）缺乏的个体在接受各种抗疟药物治疗后发生溶血的风险增加。因此，化疗研究中需要可靠的G6PD缺乏现场检测方法，并且必须评估其有效性。在布基纳法索农村地区进行的两项关于亚甲蓝（MB）抗疟治疗的II期临床试验中，对儿童和成人参与者进行了G6PD缺乏检测。将基于血红蛋白校正的烟酰胺腺嘌呤二核苷酸磷酸（NADPH）荧光试纸检测（NFP检测）结果与同样使用滤纸血样的基于聚合酶链反应（PCR）的G6PD基因分型结果进行比较。这是第一项比较这两种方法敏感性和特异性的研究。NFP检测结果与PCR结果之间具有良好的一致性。NFP检测的敏感性估计为98.2%（95.8 - 99.6%），特异性为97.1%（94.2 - 99.2%）。总之，NFP检测是在西非农村地区进行大规模G6PD缺乏筛查的一种可靠且廉价的方法。
