Anderson S A, Ostberg L G, Ehrlich P H, Kennedy R C
Department of Virology and Immunology, Southwest Foundation for Biomedical Research, San Antonio, TX 78228.
Int Immunol. 1992 Feb;4(2):135-45. doi: 10.1093/intimm/4.2.135.
Four mouse monoclonal anti-idiotypic antibodies (anti-Id) were generated against human monoclonal and polyclonal antibodies to hepatitis B surface antigen (HBsAg). These monoclonal anti-Id, along with a polyclonal anti-Id raised in rabbits, were used to characterize the idiotype (Id) specificity of the human antibody response to HBsAg (anti-HBs). The anti-Id reagents identified distinct private and cross-reactive Id expressed on monoclonal and polyclonal human anti-HBs preparations respectively. The anti-Id recognized both HBsAg combining site and non-combining site related private Id, and HBsAg combining site related cross-reactive Id. The Id specificities recognized by two of the monoclonal anti-Id were associated with the H chain alone, whereas two of the monoclonal anti-Id, along with the polyclonal anti-Id appeared to recognize Id determinants associated with both isolated H and L chains. These data suggest that Id heterogeneity exists within the human antibody response to HBsAg. The knowledge that Id heterogeneity exists is of importance in understanding the observed variability in the immune response during hepatitis B virus infection.
针对人源抗乙肝表面抗原（HBsAg）的单克隆抗体和多克隆抗体，制备了四种小鼠单克隆抗独特型抗体（抗Id）。这些单克隆抗Id，连同在兔体内产生的多克隆抗Id，被用于表征人源抗HBsAg抗体（抗-HBs）反应的独特型（Id）特异性。抗Id试剂分别鉴定出在单克隆和多克隆人源抗-HBs制剂上表达的不同的个体独特型和交叉反应独特型。抗Id识别HBsAg结合位点和非结合位点相关的个体独特型，以及HBsAg结合位点相关的交叉反应独特型。两种单克隆抗Id识别的Id特异性仅与重链相关，而另外两种单克隆抗Id以及多克隆抗Id似乎识别与分离的重链和轻链相关的Id决定簇。这些数据表明，人源抗-HBs反应中存在Id异质性。Id异质性的存在对于理解乙肝病毒感染期间观察到的免疫反应变异性具有重要意义。
