Miura Shigenobu, Arimura Tomohiro, Itoda Noriaki, Dwiarti Lies, Feng Jin Beng, Bin Cui Hong, Okabe Mitsuyasu
Tokyo Laboratories, Musashino Chemical Laboratory Ltd., 16-2 Miyamae 1-Chome, Tokyo 168-0081, Japan.
J Biosci Bioeng. 2004;97(3):153-7. doi: 10.1016/S1389-1723(04)70184-X.
The optimum temperature, initial pH, amount of added enzyme and substrate (corncob) for the hydrolysis of corncob by Acremonium cellulase were 35 degrees C, 4.5, 10 u/g-corncob and 100 g/l, respectively. Under the optimum conditions, more than 55 g/l of reducing sugars were hydrolyzed from 100 g/l of corncob to 34 g/l of glucose and 12 g/l of xylose based on dried corncob. More than 25 g/l of L-lactic acid was produced from this enzymatic hydrolyzate and less than 5 g/l of xylose remained in the 3-l airlift bioreactor. The production of L-lactic acid by simultaneous saccharification and fermentation (SSF) was also carried out in the 3-l airlift bioreactor using Acremonium thermophilus (cellulose-producer) and Rhizopus sp. MK-96-1196 (lactic acid-producer). More than 24 g/l of L-lactic acid was produced from 100 g/l of untreated raw corncob.
顶头孢霉纤维素酶水解玉米芯的最佳温度、初始pH值、添加酶量和底物（玉米芯）量分别为35℃、4.5、10 U/g玉米芯和100 g/L。在最佳条件下，基于干玉米芯，100 g/L玉米芯水解产生了超过55 g/L的还原糖，其中葡萄糖为34 g/L，木糖为12 g/L。从这种酶解产物中产生了超过25 g/L的L-乳酸，在3 L气升式生物反应器中残留的木糖少于5 g/L。还在3 L气升式生物反应器中使用嗜热顶头孢霉（纤维素生产者）和根霉MK-96-1196（乳酸生产者）通过同步糖化发酵（SSF）生产L-乳酸。100 g/L未经处理的生玉米芯产生了超过24 g/L的L-乳酸。
