de Castro Aline Machado, Ferreira Marcela Costa, da Cruz Juliana Cunha, Pedro Kelly Cristina Nascimento Rodrigues, Carvalho Daniele Fernandes, Leite Selma Gomes Ferreira, Pereira Nei
Renewable Energy Division, Research and Development Center, Petrobras, Avenue Horácio Macedo, 950 Ilha do Fundão, 21941-915 Rio de Janeiro, RJ, Brazil.
Enzyme Res. 2010 Sep 26;2010:854526. doi: 10.4061/2010/854526.
The low-cost production of cellulolytic complexes presenting high action at mild conditions and well-balanced cellulase activities is one of the major bottlenecks for the economical viability of the production of cellulosic ethanol. In the present paper, the filamentous fungus Trichoderma harzianum IOC-3844 was used for the production of cellulases from a pretreated sugarcane bagasse (namely, cellulignin), by submerged fermentation. This fungal strain produced high contents of endoglucanase activity (6,358 U·L(-1)) after 72 hours of process, and further relevant β-glucosidase and FPase activities (742 and 445 U·L(-1), resp.). The crude enzyme extract demonstrated appropriate characteristics for its application in cellulose hydrolysis, such as high thermal stability at up to 50°C, accessory xylanase activity, and absence of proteolytic activity towards azocasein. This strain showed, therefore, potential for the production of complete cellulolytic complexes aiming at the saccharification of lignocellulosic materials.
在温和条件下具有高活性且纤维素酶活性平衡的纤维素分解复合物的低成本生产，是纤维素乙醇生产经济可行性的主要瓶颈之一。在本文中，丝状真菌哈茨木霉IOC-3844被用于通过深层发酵从预处理的甘蔗渣（即纤维素木质素）生产纤维素酶。该真菌菌株在72小时的培养过程后产生了高含量的内切葡聚糖酶活性（6358 U·L(-1)），以及进一步的相关β-葡萄糖苷酶和滤纸酶活性（分别为742和445 U·L(-1)）。粗酶提取物表现出适合用于纤维素水解的特性，例如在高达50°C时具有高热稳定性、辅助木聚糖酶活性以及对偶氮酪蛋白无蛋白水解活性。因此，该菌株具有生产旨在使木质纤维素材料糖化的完整纤维素分解复合物的潜力。
