Peoples Michael C, Karnes H Thomas
Department of Pharmaceutics, Virginia Commonwealth University Medical Center, P.O. Box 980533, Richmond, VA 23298-0533, USA.
J Chromatogr B Analyt Technol Biomed Life Sci. 2005 Nov 15;827(1):5-15. doi: 10.1016/j.jchromb.2005.10.001. Epub 2005 Oct 17.
When biomolecules such as proteins, lipids, and DNA are subjected to oxidative attack by free radicals or other reactive species, a number of measurable biomarkers may be produced. The study of oxidative DNA damage is valuable in research concerning cancer and aging. The current review includes methodology involving various separation science techniques for the analysis of DNA oxidation biomarkers, mainly 8-hydroxy-2'-deoxyguanosine. This review will present recent analytical developments with respect to sample preparation and instrumental considerations, noting key outcomes and biological relevance where appropriate.
当蛋白质、脂质和DNA等生物分子受到自由基或其他活性物质的氧化攻击时，可能会产生一些可测量的生物标志物。氧化DNA损伤的研究在癌症和衰老研究中具有重要价值。本综述包括涉及各种分离科学技术分析DNA氧化生物标志物（主要是8-羟基-2'-脱氧鸟苷）的方法。本综述将介绍样品制备和仪器方面的最新分析进展，并在适当情况下指出关键结果和生物学相关性。
