Matosevic Petar, Klepac-Pulanic Tajana, Kinda Emil, Augustin Goran, Brcic Iva, Jakic-Razumovic Jasminka
Department of Surgery, University Hospital Center Zagreb, Kispaticeva 12, 10000, Zagreb, Croatia.
Gynecology Department, Community Health Center Zagreb East, Vidriceva 38, 10000, Zagreb, Croatia.
World J Surg Oncol. 2015 Aug 7;13:241. doi: 10.1186/s12957-015-0667-6.
The aim of this research was to study the levels of 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) in tumour tissue samples of colorectal carcinoma based upon immunohistochemical detection and compare those results with patients' outcome.
Tumour blocks of patients surgically treated for colorectal cancer were evaluated by 8-oxodG immunohistochemical staining. The expression was analysed in 500 tumour cells. The percentage of positive cells, as well as staining intensity, was recorded, and Allred score was calculated. For each patient, data of age, gender, tumour size and location, margin status, histologic grade, tumour stage, lymph node status, vascular invasion, overall survival, and therapy protocols were collected. Tumour grade was divided into two groups as low and high grade.
In this study, 146 consecutive patients with primary colorectal carcinoma were included. All data were available for 138 patients, and they were included in this research. There were 83 male and 55 female patients; the median age was 64 years (range 35-87 years). The results showed shorter 5- and 10-year survival in patients with 8-oxodG positive tumour cells (5-year survival, n=138, Mantel-Cox, chi-square 4.116, degree of freedom (df)=1, p<0.05; 10-year survival, n=134, Mantel-Cox, chi-square 4.374, df=1, p<0.05). The results showed a positive correlation between Allred score and high tumour grade (two-tailed Spearman's ρ 0.184; p<0.05), as well as with non-polypoid tumour growth (two-tailed Spearman's ρ 0.198; p<0.05). There was no significant difference of 8-oxodG expression related to age, sex, blood group, size and tumour site, distance from the edge of the resected tumour margin, lymph nodes involvement, and vascular invasion.
In this study, the positive correlation between 8-oxodG presence in the tumour cells, worse clinical outcome, higher tumour grade, and flat morphology was found.
本研究旨在通过免疫组化检测研究结直肠癌肿瘤组织样本中8-氧代-7,8-二氢-2'-脱氧鸟苷（8-氧代脱氧鸟苷，8-oxodG）的水平，并将这些结果与患者的预后进行比较。
对接受手术治疗的结直肠癌患者的肿瘤组织块进行8-oxodG免疫组化染色评估。在500个肿瘤细胞中分析其表达情况。记录阳性细胞百分比以及染色强度，并计算Allred评分。收集每位患者的年龄、性别、肿瘤大小和位置、切缘状态、组织学分级、肿瘤分期、淋巴结状态、血管侵犯、总生存期和治疗方案等数据。肿瘤分级分为低级别和高级别两组。
本研究纳入了146例连续性原发性结直肠癌患者。138例患者的所有数据均可用，并纳入本研究。其中男性83例，女性55例；中位年龄为64岁（范围35 - 87岁）。结果显示，8-oxodG阳性肿瘤细胞的患者5年和10年生存率较低（5年生存率，n = 138，Mantel-Cox检验，卡方值4.116，自由度(df)=1，p<0.05；10年生存率，n = 134，Mantel-Cox检验，卡方值4.374，df = 1，p<0.05）。结果显示Allred评分与高级别肿瘤（双尾Spearman秩相关系数ρ 0.184；p<0.05）以及非息肉样肿瘤生长（双尾Spearman秩相关系数ρ 0.198；p<0.05）之间呈正相关。8-oxodG表达在年龄、性别、血型、肿瘤大小和部位、距切除肿瘤边缘的距离、淋巴结受累情况和血管侵犯方面无显著差异。
本研究发现肿瘤细胞中8-oxodG的存在与较差的临床结局、较高的肿瘤分级和平坦形态之间呈正相关。
