Hsieh Ryh-Yaw, Tsai Hsuan-Ang, Syu Mei-Jywan
Division of Nephrology, Yuan General Hospital, Kaohsiung, Taiwan.
Biomaterials. 2006 Mar;27(9):2083-9. doi: 10.1016/j.biomaterials.2005.09.024. Epub 2005 Oct 17.
In this study, molecularly imprinted polymers (MIP) synthesized from two different functional monomers, beta-cyclodextrin (beta-CD) and 4-vinylpyridine (4-Vpy), were prepared. The crosslinkers used for these two monomers were epichlorohydrin (EPI) and divinylbenzene (DVB), respectively. It was attempted to adsorb the target molecule, creatinine, from its mixture solutions. A proper molar ratio of monomer/crosslinker for the preparation of the imprinted poly(beta-CD) was 1:10. Between both polymers mentioned above, the affinity of the imprinted poly(4-Vpy-co-DVB) towards creatinine was comparably superior. The imprinted poly(4-Vpy-co-DVB) for creatinine could reach a specific binding ratio of 3.11. The imprinted poly(4-Vpy-co-DVB) was further utilized to bind creatinine from human serum samples. The binding capacity of the imprinted poly(4-Vpy-co-DVB) for creatinine from the serum samples was plotted against the creatinine concentration. From the correlation, the feasibility of the imprinted poly(4-Vpy-co-DVB) thus prepared for the target analyte, creatinine, was experimentally confirmed.
在本研究中，制备了由两种不同功能单体β-环糊精（β-CD）和4-乙烯基吡啶（4-Vpy）合成的分子印迹聚合物（MIP）。用于这两种单体的交联剂分别是环氧氯丙烷（EPI）和二乙烯基苯（DVB）。尝试从其混合溶液中吸附目标分子肌酐。制备印迹聚（β-CD）的单体/交联剂的合适摩尔比为1:10。在上述两种聚合物之间，印迹聚（4-Vpy-co-DVB）对肌酐的亲和力相对较高。用于肌酐的印迹聚（4-Vpy-co-DVB）的特异性结合率可达3.11。印迹聚（4-Vpy-co-DVB）进一步用于从人血清样品中结合肌酐。将印迹聚（4-Vpy-co-DVB）对血清样品中肌酐的结合能力与肌酐浓度作图。通过相关性分析，实验证实了如此制备的印迹聚（4-Vpy-co-DVB）对目标分析物肌酐的可行性。
