Timke M, Wang-Lieu N Q, Altendorf K, Lipski A
Abteilung Mikrobiologie, Fachbereich Biologie/Chemie, Universität Osnabrück, Osnabrück, Germany.
J Appl Microbiol. 2005;99(5):1108-22. doi: 10.1111/j.1365-2672.2005.02714.x.
The microbial composition of biofilms from different locations of beer bottling plants were compared based on fatty acid profiles and correlated with the product-spoiling potential of these biofilms.
The whole cell fatty acid profiles of 78 biofilms from bottling plants of two breweries were analysed. About half of the lipid profiles were dominated by oleic and linoleic acid, which refer to a high proportion of yeasts. In addition, more than half of all samples contained dimethylacetals indicating the presence of strictly anaerobic bacteria. Typical fatty acids for potentially beer-spoiling genera were detected in three biofilms. The majority of the biofilms contained no beer-spoiling organisms, as shown by inoculation experiments in beer.
Biofilms from different locations of bottling plants were different with respect to their microbial composition. Potentially product-spoiling populations could be detected in a small number of samples.
Biofilms on industrial plants can be characterized by a fast and cultivation-independent method with respect to overall microbial composition and presence of potentially product-spoiling micro-organisms.
基于脂肪酸谱比较啤酒瓶装厂不同位置生物膜的微生物组成，并将其与这些生物膜的产品腐败潜力相关联。
分析了来自两家啤酒厂瓶装厂的78个生物膜的全细胞脂肪酸谱。约一半的脂质谱以油酸和亚油酸为主，这表明酵母比例较高。此外，超过一半的样品含有二甲基缩醛，表明存在严格厌氧菌。在三个生物膜中检测到了可能导致啤酒变质的属的典型脂肪酸。如在啤酒中的接种实验所示，大多数生物膜不含会导致啤酒变质的微生物。
瓶装厂不同位置的生物膜在微生物组成方面存在差异。在少数样品中可检测到潜在的产品腐败菌群。
对于工业装置上的生物膜，可通过一种快速且不依赖培养的方法来表征其整体微生物组成以及潜在的产品腐败微生物的存在情况。
