Takahashi T, Yamamura T, Utsunomiya J
Second Department of Surgery, Hyogo College of Medicine, Nishinomiya, Japan.
Gastroenterol Jpn. 1992 Jun;27(3):327-33. doi: 10.1007/BF02777750.
Transmural stimulation (TS; 15 V, 0.5 msec, 1-5 Hz, for 30 sec) caused a contraction in a frequency-dependent manner of the longitudinal muscle with myenteric plexus of the guinea pig ileum. Two-min premedication with human pancreatic polypeptide (HPP, 10(-8) M-10(-6) M), neuropeptide Y (NPY, 10(-8) M-10(-6) M), and peptide YY (PYY, 10(-8) M-10(-6) M) partially, by less than 35%, reduced TS (15 V, 0.5 msec, 10 Hz)-evoked contraction in a dose-dependent manner without affecting the resting tensions. Hexamethonium, phentolamine, prazocine, yohimbine, propranolol, naloxone and theophylline had no effects on the inhibitory actions of these peptides on TS-evoked contractions. TS (15 V, 0.5 msec, 10 Hz)-evoked 3H-ACh release was significantly reduced by 2-min premedication of HPP (10(-6) M), NPY (10(-6) M) and PYY (10(-6) M). The order of potency of these depressant effects on TS-evoked contraction and 3H-ACh release was PYY greater than NPY greater than HPP. It is suggested that the members of PP family have inhibitory effects on the contractile motility by depressing ACh release from myenteric plexus of the guinea pig ileum.
透壁刺激（TS；15伏，0.5毫秒，1 - 5赫兹，持续30秒）可引起豚鼠回肠含肌间神经丛的纵行肌以频率依赖的方式收缩。用人类胰多肽（HPP，10⁻⁸摩尔/升 - 10⁻⁶摩尔/升）、神经肽Y（NPY，10⁻⁸摩尔/升 - 10⁻⁶摩尔/升）和肽YY（PYY，10⁻⁸摩尔/升 - 10⁻⁶摩尔/升）进行两分钟预处理，可部分（小于35%）以剂量依赖的方式降低TS（15伏，0.5毫秒，10赫兹）诱发的收缩，且不影响静息张力。六甲铵、酚妥拉明、哌唑嗪、育亨宾、普萘洛尔、纳洛酮和茶碱对这些肽对TS诱发收缩的抑制作用无影响。用HPP（10⁻⁶摩尔/升）、NPY（10⁻⁶摩尔/升）和PYY（10⁻⁶摩尔/升）进行两分钟预处理可显著降低TS（15伏，0.5毫秒，10赫兹）诱发的³H - ACh释放。这些对TS诱发收缩和³H - ACh释放的抑制作用的效力顺序为PYY大于NPY大于HPP。提示PP家族成员通过抑制豚鼠回肠肌间神经丛释放ACh对收缩运动具有抑制作用。
