Lesniewska Violetta, Rowland Ian, Cani Patrice D, Neyrinck Audrey M, Delzenne Nathalie M, Naughton Patrick J
Northern Ireland Centre of Food and Health, University of Ulster, Coleraine, United Kingdom.
Appl Environ Microbiol. 2006 Oct;72(10):6533-8. doi: 10.1128/AEM.00915-06.
The aim of this study was to compare the effects of the mixture of Lactobacillus delbrueckii subsp. rhamnosus strain GG, Bifidobacterium lactis Bb12, and inulin on intestinal populations of lactobacilli, bifidobacteria, and enterobacteria in adult and elderly rats fed the same (in quality and quantity) diet. The portal plasma levels of two neuropeptides, neuropeptide Y (NPY) and peptide YY (PYY), were also evaluated to assess the physiological consequences of the synbiotic treatment for the gastrointestinal (GI) tracts of rats of different ages. Adult (n = 24) and elderly (n = 24) male rats were fed the AIN-93 M maintenance diet. After 2 weeks of adaptation, the diet of 12 rats of each age group was supplemented with 8% inulin and with strains GG and Bb12 to provide 2.2 x 10(9) CFU of each strain g(-1) of the diet. Blood and different regions of the GI tract were sampled from all rats after 21 days of the treatment. Treatment with the mixture of strain GG, strain BB12, and inulin induced significantly different changes in the numbers of lactobacilli, bifidobacteria, and enterobacteria of the stomach, small intestine, cecum, and colon microflora. Moreover, the GG, BB12, and inulin mixture increased the concentrations of NPY and PYY for adult rats. For the elderly animals, the PYY concentration was not changed, while the NPY concentration was decreased by treatment with the GG, BB12, and inulin mixture. The results of the present study indicate that the physiological status of the GI tract, and not just diet, has a major role in the regulation of important groups of the GI bacteria community, since even the outcome of the dietary modification with synbiotics depends on the ages of the animals.
本研究旨在比较德氏乳杆菌保加利亚亚种鼠李糖乳杆菌GG株、乳酸双歧杆菌Bb12和菊粉的混合物，对食用相同（质量和数量）饮食的成年和老年大鼠肠道中乳酸杆菌、双歧杆菌和肠杆菌数量的影响。还评估了两种神经肽，即神经肽Y（NPY）和肽YY（PYY）的门静脉血浆水平，以评估这种合生元治疗对不同年龄大鼠胃肠道（GI）的生理影响。成年（n = 24）和老年（n = 24）雄性大鼠喂食AIN-93 M维持饮食。适应2周后，每个年龄组的12只大鼠的饮食补充8%菊粉以及GG株和Bb12株，使饮食中每克含有2.2×10⁹CFU的各菌株。治疗21天后，从所有大鼠采集血液和胃肠道的不同区域样本。用GG株、BB12株和菊粉混合物进行治疗，在胃、小肠、盲肠和结肠微生物群的乳酸杆菌、双歧杆菌和肠杆菌数量上诱导了显著不同的变化。此外，GG、BB12和菊粉混合物提高了成年大鼠NPY和PYY的浓度。对于老年动物，PYY浓度没有变化，而用GG、BB12和菊粉混合物治疗使NPYYNPY浓度降低。本研究结果表明，胃肠道的生理状态，而非仅仅饮食，在调节胃肠道重要菌群方面起主要作用，因为即使是合生元饮食改良的结果也取决于动物的年龄。
