Pucher J J, Daniel J C
College of Dentistry, Department of Histology, University of Illinois, Chicago.
J Periodontol. 1992 Jun;63(6):526-32. doi: 10.1902/jop.1992.63.6.526.
The drug chlorhexidine has been widely utilized as a wound antiseptic and oral antimicrobial rinse. There have been numerous reports on its safety as an oral rinse, but its effects on wound healing have been contradictory. The present study utilized human fibroblasts derived from skin and oral tissues to test the effects of chlorhexidine on viability, growth, collagen gel contractions, and total protein synthesis. Cells were exposed for an hour to 0.005% and 0.002% chlorhexidine and for 30 seconds to 0.12% chlorhexidine. Our results indicate that a 0.002% concentration of the drug shows minimal cytotoxicity, but is able to suppress cell division almost completely. Collagen gel contraction, as a model of wound contraction, was also severely affected by all of the concentrations of chlorhexidine used. Total protein synthesis was suppressed by chlorhexidine in collagen gel culture. The data support the hypothesis that chlorhexidine is highly cytotoxic to cells in vitro, but various cell functions such as proliferation, collagen gel contraction, and protein synthesis are affected to different degrees by the drug.
洗必泰已被广泛用作伤口消毒剂和口腔抗菌漱口水。关于其作为口腔漱口水的安全性已有大量报道，但其对伤口愈合的影响却相互矛盾。本研究利用源自皮肤和口腔组织的人类成纤维细胞来测试洗必泰对细胞活力、生长、胶原凝胶收缩和总蛋白合成的影响。将细胞分别暴露于0.005%和0.002%的洗必泰中1小时，以及暴露于0.12%的洗必泰中30秒。我们的结果表明，0.002%浓度的该药物显示出最小的细胞毒性，但几乎能完全抑制细胞分裂。作为伤口收缩模型的胶原凝胶收缩，也受到所用所有浓度洗必泰的严重影响。在胶原凝胶培养中，洗必泰抑制了总蛋白合成。这些数据支持了以下假设：洗必泰在体外对细胞具有高度细胞毒性，但该药物对细胞增殖、胶原凝胶收缩和蛋白合成等各种细胞功能的影响程度不同。
