Mahale Vivek Devidas, Sharma Sonali
Department of Conservative Dentistry and Endodontics, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, Tamil Nadu, India.
J Conserv Dent Endod. 2024 Mar;27(3):252-256. doi: 10.4103/JCDE.JCDE_234_23. Epub 2024 Mar 6.
The objective of this study was to ascertain the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of royal jelly (RJ) against three microorganisms frequently linked with endodontic infections: , and .
Freshly harvested RJ was prepared at different concentrations (20%, 10%, 5%, 2.5%, and 1.25%) in distilled water. The microbial cultures of the target organisms were prepared. MIC was determined using a broth dilution technique, monitoring microbial growth. MBC was determined by inoculating agar plates with samples from tubes showing no apparent growth and evaluating the presence of bacterial or fungal growth following the incubation period.
For , the MIC and MBC were 5 mg/ml of RJ. For , the MIC and MBC were 10 mg/ml of RJ. For , both MIC and MBC were 10 mg/ml of RJ. The findings demonstrated RJ's potential to inhibit and eliminate these pathogenic microorganisms, making it a potential candidate for endodontic infection control.
The antimicrobial properties of RJ against , and present a promising avenue for enhancing infection control in endodontics. Additional investigations are needed to refine its use in clinical settings, especially in cases with mixed microbial infections.
本研究的目的是确定蜂王浆（RJ）对三种常与牙髓感染相关的微生物的最低抑菌浓度（MIC）和最低杀菌浓度（MBC）：[此处原文缺失三种微生物名称]。
将新鲜采集的RJ在蒸馏水中制备成不同浓度（20%、10%、5%、2.5%和1.25%）。制备目标微生物的培养物。使用肉汤稀释技术测定MIC，监测微生物生长。通过将无明显生长的试管中的样品接种到琼脂平板上，并在培养期后评估细菌或真菌生长的存在来确定MBC。
对于[此处原文缺失第一种微生物名称]，RJ的MIC和MBC均为5mg/ml。对于[此处原文缺失第二种微生物名称]，RJ的MIC和MBC均为10mg/ml。对于[此处原文缺失第三种微生物名称]，RJ的MIC和MBC均为10mg/ml。研究结果表明RJ具有抑制和消除这些致病微生物的潜力，使其成为牙髓感染控制的潜在候选物。
RJ对[此处原文缺失三种微生物名称]的抗菌特性为加强牙髓病感染控制提供了一条有前景的途径。需要进一步研究以完善其在临床环境中的应用，特别是在混合微生物感染的病例中。
