Chen Zhi-qiang, Wei Zhi-feng, Ye Zhang-qun, Yang Wei-min
Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Zhonghua Yi Xue Za Zhi. 2005 Jul 13;85(26):1842-4.
To explore the rationality of clinical typing of cystitis glandularis (CG) and the potential of cancerization of different types of CG.
Flow cytometry was performed to examine the ploidy of 25 fresh bladder specimens of patients with CG resected during operation, 13 high risk type and 12 low risk type, and 7 specimens of normal mucosa of bladder, by calculating the DNA index (DI). Immunohistochemistry was used to detect the expression of proliferating cell nuclear antigen (PCNA), mutant p53, p21ras, Rb, and bcl-2 in other 38 preserved specimens of CG resected during operation, 18 high risk type and 20 low risk type, and 5 specimens of normal bladder.
The DI was 1.00 +/- 0.03 in the normal bladder group, 1.05 +/- 0.07 in the high risk type CG group, and 1.01 +/- 0.05 in the low risk type CG, without significant differences between any 2 groups (t = -1.639, P = 0.115). The expression of PCNA and expression of p53 were negative in the low risk group, and PCNA was expressed in 5 specimens of high risk type CG (27.8%), and not expressed in the low risk type CG (P = 0.017). p53 was expressed in 4 specimens of high risk type CG (22.2%), and not expressed in the low risk type CG (P = 0.041). There were no significant differences in the expression of p21, Rb, and bcl-2 between the high and low risk groups.
High risk type and low risk type of CG are both benign lesions. High risk type CG may be more likely to cancerate. It is reasonable to distinguish different types of CG. p53 gene may play an important role in the canceration of CG.
探讨腺性膀胱炎（CG）临床分型的合理性及不同类型CG的癌变潜能。
采用流式细胞术检测25例手术切除的CG患者新鲜膀胱标本（高危型13例，低危型12例）及7例正常膀胱黏膜标本的倍体，计算DNA指数（DI）。应用免疫组织化学法检测另外38例手术切除的CG保存标本（高危型18例，低危型20例）及5例正常膀胱标本中增殖细胞核抗原（PCNA）、突变型p53、p21ras、Rb和bcl-2的表达。
正常膀胱组DI为1.00±0.03，高危型CG组为1.05±0.07，低危型CG组为1.01±0.05，任意两组间差异均无统计学意义（t=-1.639，P=0.115）。低危组PCNA及p53表达均为阴性，高危型CG有5例标本表达PCNA（27.8%），低危型CG无表达（P=0.017）。高危型CG有4例标本表达p53（22.2%），低危型CG无表达（P=0.041）。高危组与低危组p21、Rb及bcl-2表达差异均无统计学意义。
CG高危型和低危型均为良性病变。高危型CG可能更易癌变。区分不同类型的CG是合理的。p53基因可能在CG癌变中起重要作用。
