Löffler Frank E, Sanford Robert A, Ritalahti Kirsti M
School of Civil and Environmental Engineering, Georgia Institute of Technology, Atlanta 30332-0512, USA.
Methods Enzymol. 2005;397:77-111. doi: 10.1016/S0076-6879(05)97005-5.
Strategies and procedures for enriching, isolating, and cultivating reductively dechlorinating bacteria that use chloroorganic compounds as metabolic electron acceptors from environmental samples are described. Further, nucleic acid-based approaches used to detect and quantify dechlorinator (i.e., Dehalococcoides)-specific genes are presented.
本文描述了从环境样本中富集、分离和培养以氯代有机化合物作为代谢电子受体的还原性脱氯细菌的策略和程序。此外，还介绍了用于检测和定量脱氯剂（即脱卤球菌属）特异性基因的基于核酸的方法。
