中性pH条件下铁氧化细菌的富集与分离

Enrichment and isolation of iron-oxidizing bacteria at neutral pH.

作者信息

Emerson David, Floyd Melissa Merrill

机构信息

American Type Culture Collection, Manassas, Virginia 20110, USA.

出版信息

Methods Enzymol. 2005;397:112-23. doi: 10.1016/S0076-6879(05)97006-7.

DOI:10.1016/S0076-6879(05)97006-7

PMID:16260287

Abstract

Methods are provided for the culture of neutrophilic Fe-oxidizing bacteria (FeOB) that grow under microaerobic and anaerobic conditions. For oxygen-requiring lithotrophic Fe oxidizers, it is essential that both O2 and Fe(II) concentrations are kept low, but that an adequate flux of both O2 and Fe(II) are provided to support growth. Techniques using opposing gradients of Fe(II) and O2 are discussed. Preparation of stock solutions of FeS and FeCl2 are described. Methods for the culture of anaerobic FeOB that utilize nitrate or light (anoxygenic photosynthesis) are discussed and presented briefly.

摘要

提供了用于培养在微需氧和厌氧条件下生长的嗜中性铁氧化细菌（FeOB）的方法。对于需氧的无机营养型铁氧化剂，必须将O2和Fe(II)的浓度都保持在较低水平，但要提供足够的O2和Fe(II)通量以支持生长。讨论了使用Fe(II)和O2相反梯度的技术。描述了FeS和FeCl2储备溶液的制备方法。简要讨论并介绍了利用硝酸盐或光（无氧光合作用）培养厌氧FeOB的方法。