Lioubavina-Hack Natalia, Karring Thorkild, Lynch Samuel E, Lindhe Jan
Department of Periodontology and Oral Gerontology, Royal Dental College, University of Aarhus, Denmark.
J Clin Periodontol. 2005 Dec;32(12):1247-53. doi: 10.1111/j.1600-051X.2005.00791.x.
To evaluate whether bone formation under Teflon capsules may be enhanced by concomitant implantation of recombinant human platelet-derived growth factor-BB/insulin-like growth factor-I (rhPDGF-BB/IGF-I) incorporated into a methyl cellulose gel.
Fifty-five male 6-month-old albino rats of the Wistar strain were used in the study. The lateral aspect of the mandibular ramus was exposed on both sides of the jaw. In 70 sites, the periosteum was removed from the ramus, leaving the bone denuded, while in 35 sites, it was preserved. On 10 non-periosteal (P-) sites and five periosteal (P+) sites, an empty rigid teflon capsule (d=7 mm), serving as control, was placed on the ramus. In the 40 test animals, the capsule placed on the one side of the jaw was filled at random with one of three different concentrations (1,200, 600, 150 microg/ml) of rhPDGF-BB/IGF-I gel. The capsules placed on the contralateral side of the jaw contained a placebo methyl cellulose gel. Each growth factor group, defined according to the gel concentration, and the placebo group contained 10 capsules placed on the P- side and five capsules placed on the P+ side. Two months after surgery, all animals were sacrificed.
Histologic analysis revealed that in the non-filled control capsules, the amount of new bone including the bone marrow was 29.9% and 39.7% of the capsule area on the P- and P+ sides, respectively. In the test capsules with the growth factor gel and placed on the P-sides, the amounts of new bone ranged from 5.6% to 6.3%, which were similar (p>0.05) to that formed in the capsules filled with the methyl cellulose gel (5.5%). New bone formation was larger in the capsules on the P+ sides than in those on the P- sides but was similar in the capsules with different growth factor concentrations (range 17.9-19.6%) and in those with placebo gel (21.0%). In all groups, the carrier gel was poorly absorbed and occupied most of the capsules.
Local application of a methyl cellulose gel obstructed bone formation under Teflon capsules placed adjacent to uninjured cortical bone in the mandibular ramus of rats. These data suggest that another material should be utilized to deliver growth factors under Teflon membranes for guided bone regeneration.
评估将重组人血小板源性生长因子-BB/胰岛素样生长因子-I（rhPDGF-BB/IGF-I）掺入甲基纤维素凝胶中并同时植入，是否能增强聚四氟乙烯胶囊下的骨形成。
本研究使用了55只6月龄的雄性Wistar白化大鼠。暴露双侧下颌支的外侧。在70个部位，从下颌支去除骨膜，使骨面裸露，而在35个部位保留骨膜。在10个非骨膜（P-）部位和5个骨膜（P+）部位，将一个空的刚性聚四氟乙烯胶囊（直径 = 7毫米）作为对照放置在下颌支上。在40只实验动物中，放置在下颌一侧的胶囊随机填充三种不同浓度（1200、600、150微克/毫升）的rhPDGF-BB/IGF-I凝胶中的一种。放置在下颌对侧的胶囊含有安慰剂甲基纤维素凝胶。根据凝胶浓度定义的每个生长因子组和安慰剂组，在P-侧放置10个胶囊，在P+侧放置5个胶囊。术后两个月，处死所有动物。
组织学分析显示，在未填充的对照胶囊中，包括骨髓在内的新骨量在P-侧和P+侧分别占胶囊面积的29.9%和39.7%。在放置于P-侧且填充有生长因子凝胶的实验胶囊中，新骨量在5.6%至6.3%之间，与填充甲基纤维素凝胶的胶囊中形成的新骨量（5.5%）相似（p>0.05）。P+侧胶囊中的新骨形成比P-侧的大，但在不同生长因子浓度的胶囊（范围为17.9 - 19.6%）和含有安慰剂凝胶的胶囊（21.0%）中相似。在所有组中，载体凝胶吸收不良，占据了大部分胶囊。
局部应用甲基纤维素凝胶阻碍了放置在大鼠下颌支未受伤皮质骨旁的聚四氟乙烯胶囊下的骨形成。这些数据表明，应使用另一种材料在聚四氟乙烯膜下递送生长因子以促进引导性骨再生。
