Kostopoulos L, Karring T
Department of Periodontology and Oral Gerontology, Royal Dental College, Faculty of Health Sciences, University of Aarhus, Denmark.
J Clin Periodontol. 1995 Mar;22(3):247-54. doi: 10.1111/j.1600-051x.1995.tb00142.x.
The aim of the present investigation was to evaluate the bone-forming capacity of the outer (fibrous) and inner (cambium) layer of the mandibular periosteum in skeletally mature rats. The experiment was carried out in 25 rats. The mandibular ramus was exposed on one side (experimental side) after elevation of a muscle-periosteal flap. A teflon capsule was placed with its opening facing the periosteum at the subsurface of the raised muscle-periosteal flap after suturing. In the contralateral side serving as control, the periosteum of the lateral aspect of the mandibular ramus was left intact. This time the teflon capsule was placed with its opening facing the periosteum left behind at the ramus. The histological analysis demonstrated that in all experimental and control specimens, some bone was produced at 7 days after operation. In the experimental specimens, however, both the incidence and the amount of newly-formed bone in the teflon capsules gradually decreased from 7 to 120 days, while increasing in the control capsules placed over the periosteum. At 120 days, the mean amount of new bone produced in the experimental capsules was 3% (range 0-15%) of the total space created by the capsule, while it was 68% (range 41-85%) for the control capsules. The results demonstrated that substantial amounts of bone can be produced predictably by the placement of an occlusive teflon capsule facing mandibular covered with periosteum. Bone produced from the periosteum and without continuity with existing bone becomes resorbed with time.
本研究的目的是评估骨骼成熟大鼠下颌骨骨膜外层（纤维层）和内层（形成层）的成骨能力。实验在25只大鼠身上进行。掀起肌肉-骨膜瓣后，在一侧（实验侧）暴露下颌支。缝合后，将一个特氟龙胶囊开口朝向骨膜放置在掀起的肌肉-骨膜瓣下方。在作为对照的对侧，下颌支外侧的骨膜保持完整。此次将特氟龙胶囊开口朝向留在下颌支上的骨膜放置。组织学分析表明，在所有实验和对照标本中，术后7天均有一些骨形成。然而，在实验标本中，特氟龙胶囊内新形成骨的发生率和数量在术后7天至120天逐渐减少，而放置在骨膜上的对照胶囊内新形成骨的发生率和数量则增加。在120天时，实验胶囊内新形成骨的平均量占胶囊所形成总空间的3%（范围为0 - 15%），而对照胶囊为68%（范围为41 - 85%）。结果表明，通过将封闭的特氟龙胶囊放置在覆盖有骨膜的下颌骨上，可以可预测地产生大量的骨。由骨膜产生且与现有骨无连续性的骨会随着时间而被吸收。
