Marston A, Hostettmann K
Laboratoire de Pharmacognosie et Phytochimie, Université de Genéve, Quai Ernest-Ansermet 30, CH-1211 Genéve 4, Switzerland.
J Chromatogr A. 2006 Apr 21;1112(1-2):181-94. doi: 10.1016/j.chroma.2005.10.018. Epub 2005 Nov 2.
Counter-current chromatography is a very versatile separation technique which does not require a solid stationary phase. It relies simply on the partition of a sample between the two phases of an immiscible solvent system. Some of the more recent applications of the method to the separation of plant-derived natural products are described here. Crude plant extracts and semi-pure fractions can be chromatographed, with sample loads ranging from milligrams to grams. Aqueous and non-aqueous solvent systems are used and the separation of compounds with a wide range of polarities is possible. The technique is complementary to other chromatographic methods and is compatible with gradient systems. The possibilities for solvent selection are almost limitless but some guidelines for the choice of successful systems are presented.
逆流色谱法是一种非常通用的分离技术，它不需要固体固定相。它仅依赖于样品在不混溶溶剂系统的两相之间的分配。本文介绍了该方法在分离植物源天然产物方面的一些最新应用。粗植物提取物和半纯级分都可以进行色谱分析，样品负载量从毫克到克不等。使用水相和非水相溶剂系统，可以分离各种极性的化合物。该技术是其他色谱方法的补充，并且与梯度系统兼容。溶剂选择的可能性几乎是无限的，但本文给出了一些成功选择溶剂系统的指导原则。
