Baglam T, Sari M, Mine Yazici Z, Yuksel M, Uneri C
Department of Otolaryngology Head Neck Surgery, School of Medicine, Gaziantep University, Gaziantep, Turkey.
J Laryngol Otol. 2010 Oct;124(10):1091-4. doi: 10.1017/S0022215110000988. Epub 2010 May 20.
This study aimed to evaluate the presence of reactive oxygen species in laryngeal cancer tissue, using a luminol-amplified chemiluminescence method.
Fourteen patients with histopathologically diagnosed laryngeal squamous cell carcinoma were enrolled. Patients with recurrent tumours or a history of prior chemotherapy or radiotherapy were excluded. Tissue specimens were harvested both from the tumour itself and from the neighbouring, apparently normal mucosa (immediately after tumour removal). Tissue specimens were washed with ice-cold saline solution and processed immediately, without storage. The level of reactive oxygen species was measured quantitatively by a luminol-amplified chemiluminescence method.
The mean luminol-amplified chemiluminescence values for tumour and control tissue were 140.52 (standard error of the mean 40.21) and 121.36 (standard error of the mean 35.33) relative light units/mg tissue, respectively. Furthermore, mean tumour and control luminol chemiluminescence values were compared for stage one and two tumours versus stage three and four tumours. Both the tumour and the control luminol chemiluminescence values for the latter tumour group were significantly higher than those for the former tumour group.
This study measured directly the levels of reactive oxygen species in samples of laryngeal cancer tissue and normal mucosa. Higher levels of reactive oxygen species were found in laryngeal cancer tissue, suggesting a relationship between reactive oxygen species and laryngeal cancer.
本研究旨在使用鲁米诺增强化学发光法评估喉癌组织中活性氧的存在情况。
纳入14例经组织病理学诊断为喉鳞状细胞癌的患者。排除复发性肿瘤患者或有化疗或放疗史的患者。在肿瘤切除后立即从肿瘤本身及其相邻的看似正常的黏膜获取组织标本。组织标本用冰冷的盐溶液冲洗后立即进行处理，不进行储存。采用鲁米诺增强化学发光法定量测定活性氧水平。
肿瘤组织和对照组织的平均鲁米诺增强化学发光值分别为140.52相对光单位/毫克组织（平均标准误40.21）和121.36相对光单位/毫克组织（平均标准误35.33）。此外，比较了一期和二期肿瘤与三期和四期肿瘤的肿瘤和对照鲁米诺化学发光平均值。后一组肿瘤的肿瘤和对照鲁米诺化学发光值均显著高于前一组肿瘤。
本研究直接测量了喉癌组织和正常黏膜样本中的活性氧水平。在喉癌组织中发现较高水平的活性氧，提示活性氧与喉癌之间存在关联。
