Coutinho C C, Silva J T, Panek A D
Departamento de Bioquímica, Universidade Federal do Rio de Janeiro, Brasil.
Biochem Int. 1992 Mar;26(3):521-30.
Trehalase activity decreased in 95% at the onset of the transition phase of growth of S. cerevisiae. The question which we raised was whether this phenomenon was due to proteolysis or to conversion of the enzyme to a less active form (dephosphorylation). Immunological methods allowed to identify the presence of the trehalase protein during cell growth. At the same stage of growth, an increase in the non-phosphorylated enzyme was detected "in vitro". Results utilizing mutant strains also indicated that regulation occurred by interconversion of forms. The same mechanism also seems to control trehalase activity in non proliferating conditions.
在酿酒酵母生长的转变阶段开始时，海藻糖酶活性降低了95%。我们提出的问题是，这种现象是由于蛋白水解作用还是由于酶转化为活性较低的形式（去磷酸化）。免疫方法能够鉴定细胞生长过程中海藻糖酶蛋白的存在。在相同的生长阶段，“体外”检测到非磷酸化酶有所增加。利用突变菌株得到的结果也表明，调节是通过不同形式之间的相互转化发生的。相同的机制似乎也在非增殖条件下控制着海藻糖酶的活性。
