G尾端粒人肽抗体：人类单链端粒悬垂的简易测量方法。
G-tail telomere HPA: simple measurement of human single-stranded telomeric overhangs.
作者信息
Tahara Hidetoshi, Kusunoki Mayuko, Yamanaka Yusuke, Matsumura Shusaku, Ide Toshinori
机构信息
Department of Cellular and Molecular Biology, Division of Integrated Medical Science, Graduate School of Biomedical Sciences, Hiroshima University, Kasumi 1-2-3, Minami-ku, Hiroshima City, Hiroshima 734-8551, Japan.
出版信息
Nat Methods. 2005 Nov;2(11):829-31. doi: 10.1038/nmeth797.
Accurate measurement of telomeric 3'-overhang (G-tail) lengths is essential for investigation of the biological effects of telomere dysfunction. G-tail telomere hybridization protection assay (Gt-telomere HPA) has the advantages of being simple to perform, accurate and highly sensitive for G tails as short as 20 nucleotides. Furthermore, Gt-telomere HPA is specific and quantitative for human G tails, and can be used to assay cell lysates as well as genomic DNA.
准确测量端粒3'-末端悬垂（G尾）长度对于研究端粒功能障碍的生物学效应至关重要。G尾端粒杂交保护分析（Gt-端粒HPA）具有操作简单、对短至20个核苷酸的G尾准确且高度灵敏的优点。此外，Gt-端粒HPA对人类G尾具有特异性和定量性，可用于检测细胞裂解物以及基因组DNA。
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