Martínez-Checa Fernando, Béjar Victoria, Llamas Inmaculada, Del Moral Ana, Quesada Emilia
Microbial Exopolysaccharide Research Group, Department of Microbiology, Faculty of Pharmacy, Cartuja Campus, University of Granada, 18071 Granada, Spain.
Int J Syst Evol Microbiol. 2005 Nov;55(Pt 6):2385-2390. doi: 10.1099/ijs.0.63809-0.
Strain F-32T, which produces exopolysaccharides and contains polyunsaturated fatty acids, was isolated from a hypersaline water sample collected from Fuente de Piedra (southern Spain). Phylogenetic analyses indicated conclusively that the strain in question belonged to the genus Alteromonas. Phenotypic tests showed that it could be assigned to the genus Alteromonas although it had a number of distinctive characteristics: it is moderately halophilic, growing best with 7.5-10 % w/v NaCl; it grows at 4 degrees C and produces H2S; it does not grow with d-cellobiose, d-fructose, d-galactose, d-glucose or lactose as sole sources of carbon and energy; and its fatty-acid profile is typical of Alteromonas but it also contains a large amount of an unusual acid with three double bonds [18 : 3omega6c (6, 9, 12); 5.01 %, w/v]. The major isoprenoid quinone is Q8. The DNA G+C content is 46.3 mol%. The phylogenetic, phenotypic and genetic properties of strain F-32T place it within a novel species, for which the name Alteromonas hispanica sp. nov. is proposed. The type strain is F-32T (=CECT 7067T=LMG 22958T).
菌株F-32T从西班牙南部富恩特德皮德拉采集的高盐水样中分离得到，它能产生胞外多糖并含有多不饱和脂肪酸。系统发育分析最终表明，该菌株属于交替单胞菌属。表型测试表明，尽管它有一些独特的特征，但仍可归为交替单胞菌属：它是中度嗜盐菌，在7.5 - 10% w/v NaCl条件下生长最佳；能在4℃生长并产生H₂S；不能以d-纤维二糖、d-果糖、d-半乳糖、d-葡萄糖或乳糖作为唯一碳源和能源生长；其脂肪酸谱是交替单胞菌属的典型特征，但也含有大量具有三个双键的不寻常酸[18 : 3ω6c (6, 9, 12)；5.01%，w/v]。主要类异戊二烯醌为Q8。DNA G+C含量为46.3 mol%。菌株F-32T的系统发育、表型和遗传特性表明它属于一个新物种，为此提议将其命名为西班牙交替单胞菌（Alteromonas hispanica sp. nov.）。模式菌株为F-32T（=CECT 7067T=LMG 22958T）。
