荧光引导光学相干断层扫描在大鼠模型中用于早期膀胱癌的诊断
Fluorescence guided optical coherence tomography for the diagnosis of early bladder cancer in a rat model.
作者信息
Wang Z G, Durand D B, Schoenberg M, Pan Y T
机构信息
Department of Biomedical Engineering, State University of New York at Stony Brook, Stony Brook, NY 11794, USA.
出版信息
J Urol. 2005 Dec;174(6):2376-81. doi: 10.1097/01.ju.0000180413.98752.a1.
PURPOSE
We describe the technique of fluorescence image guided optical coherence tomography (FG-OCT). We examined its ability to enhance specificity and sensitivity for the noninvasive diagnosis of early bladder cancer.
MATERIALS AND METHODS
Transitional cell carcinoma was developed in 54 Fisher 344 female rats by intravesical methyl-nitroso-urea instillations. Two or three rats were diagnosed sequentially by 5-ALA (5-aminolevulinic acid hydrochloride) induced fluorescence imaging, cross-sectional OCT and histological microscopy weekly during weeks 11 to 33 following initial methyl-nitroso-urea instillation to track the course of carcinogenesis.
RESULTS
The specificity of fluorescence detection was significantly enhanced by FG-OCT (53% and 93%, respectively, p <0.0001). The sensitivity of fluorescence detection and FG-OCT was 79% and 100%, respectively.
CONCLUSIONS
FG-OCT cystoscopy has the potential to diagnose early bladder cancer with high sensitivity and specificity with drastically decreased imaging time compared to that of white light guided OCT cystoscopy.
目的
我们描述了荧光图像引导光学相干断层扫描（FG-OCT）技术。我们研究了其在早期膀胱癌无创诊断中提高特异性和敏感性的能力。
材料与方法
通过膀胱内灌注甲基亚硝基脲在54只Fisher 344雌性大鼠中诱发移行细胞癌。在首次灌注甲基亚硝基脲后的第11至33周，每周依次对两到三只大鼠进行5-氨基乙酰丙酸（5-ALA，盐酸5-氨基乙酰丙酸）诱导的荧光成像、横截面OCT和组织学显微镜检查，以追踪致癌过程。
结果
FG-OCT显著提高了荧光检测的特异性（分别为53%和93%，p<0.0001）。荧光检测和FG-OCT的敏感性分别为79%和100%。
结论
与白光引导的OCT膀胱镜检查相比，FG-OCT膀胱镜检查有可能以高敏感性和特异性诊断早期膀胱癌，且成像时间大幅缩短。
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